2, 6-Diacetyl-4-C-(β-D-glucopyranosyl)-4-hydroxycyclohexane-1, 3, 5-trione was obtained by the deacetylation of 2, 6-diacetyl-4-C-(2, 3, 4, 6-tetra-O-acetyl-β-D-glucopyranosyl)-4- hydroxycyclohexane-1, 3, 5-trione which prepared by the C-glucosylation of 2, 6-diacetyl-1, 3, 4, 5-benzenetetrol with 2, 3, 4, 6-tetra-O-acetyl-α-D-glucopyranosyl bromide. The acid hydrolysis of this new C-glucoside was investigated.