A new LC-MS/MS method for the quantification of endogenous and vinyl chloride-induced 7-(2-Oxoethyl) guanine in Sprague–Dawley rats

…, PB Upton, G Hatch, D Winsett, P Evansky…

Index: Mutlu, Esra; Jeong, Yo-Chan; Collins, Leonard B.; Ham, Amy-Joan L.; Upton, Patricia B.; Hatch, Gary; Winsett, Darrell; Evansky, Paul; Swenberg, James A. Chemical Research in Toxicology, 2012 , vol. 25, # 2 p. 391 - 399

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Citation Number: 6

Abstract

Vinyl chloride (VC) is an industrial chemical that is known to be carcinogenic to animals and humans. VC primarily induces hepatic angiosarcomas following high exposures (≥ 50 ppm). VC is also found in Superfund sites at ppb concentrations as a result of microbial metabolism of trichloroethylene and perchloroethylene. Here, we report a new sensitive LC- MS/MS method to analyze the major DNA adduct formed by VC, 7-(2-oxoethylguanine)(7- ...