Dependence of effective molarity on linker length for an intramolecular protein-ligand system

VM Krishnamurthy, V Semetey, PJ Bracher…

Index: Krishnamurthy, Vijay M.; Semetey, Vincent; Bracher, Paul J.; Shen, Nan; Whitesides, George M. Journal of the American Chemical Society, 2007 , vol. 129, # 5 p. 1312 - 1320

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Citation Number: 175

Abstract

This paper reports dissociation constants and “effective molarities”(M eff) for the intramolecular binding of a ligand covalently attached to the surface of a protein by oligo (ethylene glycol)(EG n) linkers of different lengths (n= 0, 2, 5, 10, and 20) and compares these experimental values with theoretical estimates from polymer theory. As expected, the value of M eff is lowest when the linker is too short (n= 0) to allow the ligand to bind ...