Synthesis and use of isotope-labelled substrates for a mechanistic study on human α-methylacyl-CoA racemase 1A (AMACR; P504S)

…, AD Wilson, TJ Woodman, MD Threadgill…

Index: Darley, Daniel J.; Butler, Danica S.; Prideaux, Samuel J.; Thornton, Thomas W.; Wilson, Abigail D.; Woodman, Timothy J.; Threadgill, Michael D.; Lloyd, Matthew D. Organic and Biomolecular Chemistry, 2009 , vol. 7, # 3 p. 543 - 552

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Citation Number: 28

Abstract

α-Methylacyl-CoA racemase (AMACR) is an important enzyme for the metabolism of branched-chain lipids and drugs. The enzyme is over-expressed in prostate and other cancers. AMACR 1A, the major splice variant, was purified from recombinant E. colicells as a His-tag protein. Purified enzyme catalysed chiral inversion of both S-and R-2- methyldecanoyl-CoA, with an equilibrium constant of 1.09±0.14 (2S/2R). Reactions with ...