Enzyme-purification and catalytic transformations in a microstructured PASS flow reactor using a new tyrosine-based Ni-NTA linker system attached to a …

G Dräger, C Kiss, U Kunz, A Kirschning

Index: Draeger, Gerald; Kiss, Csilla; Kunz, Ulrich; Kirschning, Andreas Organic and Biomolecular Chemistry, 2007 , vol. 5, # 22 p. 3657 - 3664

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Citation Number: 43

Abstract

The synthesis of a Ni-nitrilotriacetic acid (Ni-NTA) attached via a new tyrosine-based linker matrix on monolithic crosslinked poly (vinyl benzyl chloride)/poly (vinylpyrrolidinone) is described. This matrix is incorporated inside a microstructured PASSflow reactor which was used for automatic purification and immobilisation of His6-tagged proteins. These could be used as stable and highly active biocatalysts for the synthesis of (R)-benzoin (6),(R)-2- ...