Cross-linked aggregates of (R)-oxynitrilase: a stable, recyclable biocatalyst for enantioselective hydrocyanation

LM Van Langen, RP Selassa, F Van Rantwijk…

Index: Van Langen, Luuk M.; Selassa, Rhoderick P.; Van Rantwijk, Fred; Sheldon, Roger A. Organic Letters, 2005 , vol. 7, # 2 p. 327 - 329

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Citation Number: 86

Abstract

The (R)-oxynitrilase from almonds was immobilized as a cross-linked enzyme aggregate (CLEA) via precipitation with 1, 2-dimethoxyethane and subsequent cross-linking using glutaraldehyde. The resulting preparation was a highly effective hydrocyanation catalyst under microaqueous conditions, which suppress the nonenzymatic background reaction. The beneficial effect of these latter conditions on the hydrocyanation of slow-reacting ...