Directed evolution to investigate steric control of enzymatic oxidosqualene cyclization. An isoleucine-to-valine mutation in cycloartenol synthase allows lanosterol and …

…, L Hua, LB Darr, WK Wilson, J Pang…

Index: Hart, Elizabeth A.; Hua, Ling; Darr, Lisa B.; Wilson, William K.; Pang, Jihai; Matsuda, Seiichi P. T. Journal of the American Chemical Society, 1999 , vol. 121, # 42 p. 9887 - 9888

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Citation Number: 103

Abstract

Oxidosqualene cyclases convert (S)-2, 3-oxidosqualene to triterpene alcohols by protonation, cyclization, rearrangement, and elimination reactions. This catalytic approach has great plasticity and produces a large family of compounds; 83 different natural products are C30H50O isomers structurally consistent with being enzymatically rearranged products of oxidosqualene. 1, 2 Two rigorously conserved Saccharomyces cereVisiae lanosterol ...