Sesquiterpene synthases Cop4 and Cop6 from Coprinus cinereus: catalytic promiscuity and cyclization of farnesyl pyrophosphate geometric isomers

F Lopez??Gallego, SA Agger, D Abate??Pella…

Index: Lopez-Gallego, Fernando; Agger, Sean A.; Abate-Pella, Daniel; Distefano, Mark D.; Schmidt-Dannert, Claudia ChemBioChem, 2010 , vol. 11, # 8 p. 1093 - 1106

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Citation Number: 38

Abstract

Abstract Sesquiterpene synthases catalyze with different catalytic fidelity the cyclization of farnesyl pyrophosphate (FPP) into hundreds of known compounds with diverse structures and stereochemistries. Two sesquiterpene synthases, Cop4 and Cop6, were previously isolated from Coprinus cinereus as part of a fungal genome survey. This study investigates the reaction mechanism and catalytic fidelity of the two enzymes. Cyclization of all-trans- ...