Aromatic l-Amino Acid Decarboxylase from Micrococcus percitreus Purification, Crystallization and Properties

H Nakazawa, H Kumagai, H Yamada

Index: Nakazawa, Hidetsugu; Kumagai, Hidehiko; Yamada, Hideaki Agricultural and Biological Chemistry, 1981 , vol. 45, # 11 p. 2543 - 2552

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Citation Number: 11

Abstract

An aromatic l-amino acid decarboxylase was crystallized from the cell free extract of Micrococcus percitreus. The purification procedure included protamine sulfate treatment, ammonium sulfate fractionation, DEAE-Sephadex column chromatography and Sephadex G- 200 filtration. Crystals were obtained from a solution of the purified enzyme by the addition of ammonium sulfate. The crystalline enzyme preparation was homogeneous as judged by ...

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