Abstract Using a cell-based high-throughput screen, we identified isoxazolo [5, 4-d] pyrimidines as novel small-molecule correctors of the cystic fibrosis mutant protein ΔF508- CFTR. 22 Isoxazolo [5, 4-d] pyrimidine analogues were synthesized and tested. Synthesis of the key intermediate, 5-amino-3-arylisoxazole-4-carboxamide, was accomplished by nitrile oxide cycloaddition to (2-amino-1-cyano-2-oxoethyl) sodium. Formation of 3-arylisoxazolo ...
![3-(4-chlorophenyl)-2H-[1,2]oxazolo[5,4-d]pyrimidin-4-one Structure](https://image.chemsrc.com/caspic/422/15783-73-0.png)
