Cytochrome P-450-LM3, 4 has been found to catalyze intramolecular amidation (1-2), as evidenced by multiple turnovers (2.2) of the enzyme under optimal condition^.'^ The reaction rate, as determined by product 2 formation,'3b is linearly dependent on P-450 concentration (0-1 MM), time (0-3 min), and substrate (1) concentration (0-0.53 mM), with a turnover number14 of 1.0 for the LM3, 4 isozyme mixture, a value that is a lower limit since ...
