Chemical research in toxicology

Analysis of peroxynitrite reactions with guanine, xanthine, and adenine nucleosides by high-pressure liquid chromatography with electrochemical detection: C8- …

RS Sodum, ES Fiala

Index: Sodum, Rama S.; Fiala, Emerich S. Chemical Research in Toxicology, 2001 , vol. 14, # 4 p. 438 - 450

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Citation Number: 38

Abstract

Peroxynitrite, the reaction product of nitric oxide and superoxide anion, and a powerful oxidant, was found to nitrate as well as oxidize adenine, guanine, and xanthine nucleosides. A highly sensitive reverse-phase HPLC method with a dual-mode electrochemical detector, which reduces the nitro product at the first electrode and detects the reduced product by oxidation at the second electrode, was applied to detect femtomole levels of 8- ...