Mechanism-based affinity capture of sirtuins

Y Cen, JN Falco, P Xu, DY Youn…

Index: Organic and Biomolecular Chemistry, , vol. 9, # 4 p. 987 - 993

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Citation Number: 14

Abstract

... low predicted pK a , 6–7, allowing us to cross link NAD + to biotin at neutral to slightly acidic pH, where chemistry on sirtuins ... 'Sirtuin capture' using the described chemical tools was ... Reactions were typically performed in 300 mM phosphate buffer pH 7.5 in a total volume of 50 μL ...