Culture medium containing 200 μ m O-dinitrobenzene suppressed cell growth of Issatchenkia orientalis for about 24 hr and after a lag phase, the cells began to increase with concomitant metabolism of O-dinitrobenzene by glutathione conjugation. The resulting glutathione conjugate, S-(2-nitrophenyl) glutathione, was further enzymatically metabolized and released into the growth medium. The final metabolite was identified as S-(2- ...