Use of 'small but smart'libraries to enhance the enantioselectivity of an esterase from Bacillus stearothermophilus towards tetrahydrofuran??3??yl acetate

…, MG Gall, IV Pavlidis, ML Thompson, M Schmidt…

Index: Nobili, Alberto; Gall, Markus G.; Pavlidis, Ioannis V.; Thompson, Mark L.; Schmidt, Marlen; Bornscheuer, Uwe T. FEBS Journal, 2013 , vol. 280, # 13 p. 3084 - 3093

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Citation Number: 14

Abstract

Abstract Two libraries of simultaneous double mutations in the active site region of an esterase from Bacillus stearothermophilus were constructed to improve the enantioselectivity in the hydrolysis of tetrahydrofuran-3-yl acetate. As screening of large mutant libraries is hampered by the necessity for GC/MS analysis, mutant libraries were designed according to a 'small but smart'concept. The design of focused libraries was ...