Aspergillus nidulansα??galactosidase of glycoside hydrolase family 36 catalyses the formation of α??galacto??oligosaccharides by transglycosylation

H Nakai, MJ Baumann, BO Petersen, Y Westphal…

Index: Nakai, Hiroyuki; Baumann, Martin J.; Petersen, Bent O.; Westphal, Yvonne; Hachem, Maher Abou; Dilokpimol, Adiphol; Duus, Jens .; Schols, Henk A.; Svensson, Birte FEBS Journal, 2010 , vol. 277, # 17 p. 3538 - 3551

Full Text: HTML

Citation Number: 24

Abstract

Abstract The α-galactosidase from Aspergillus nidulans (AglC) belongs to a phylogenetic cluster containing eukaryotic α-galactosidases and α-galacto-oligosaccharide synthases of glycoside hydrolase family 36 (GH36). The recombinant AglC, produced in high yield (0.65 g· L− 1 culture) as His-tag fusion in Escherichia coli, catalysed efficient transglycosylation with α-(1→ 6) regioselectivity from 40 mm 4-nitrophenol α-d-galactopyranoside, melibiose ...