Carbohydrate Research

Regioselective synthesis of trisaccharides by use of a reversed hydrolysis activity of α-and β-d-galactosidase

K Ajisaka, H Fujimoto

Index: Ajisaka, Katsumi; Fujimoto, Hiroshi Carbohydrate Research, 1989 , vol. 185, p. 139 - 146

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Citation Number: 52

Abstract

Abstract Incubation of a solution containing d-galactose and a high concentration of sucrose in the presence of α-d-galactosidase from M. vinacea afforded raffinose and planteose in a ratio of∼ 3: 2. On the other hand, circulation of a solution of d-galactose and sucrose through a reaction system consisting of columns, in series, of immobilized α-d-galactosidase and activated carbon gave only raffinose, eluted from the activated carbon column in a ...