The infrared study of the aromatic components of hops (Humulus lupulus) cuticular wax was performed. HATR FT-IR technique for fresh leaves and their extract analysis was applied. Phenylmethyl myristate, 2-phenylethyl myristate and docosyl benzoate were synthesized and used as reference standards. An absorption band in the range of 709–966cm− 1 indicates the presence of aromatic esters in plant cuticular waxes.