Here we present a photodegradable hydrogel as a biocompatible, nonfouling photoresist capable of presenting positive and negative features through single-photon and two-photon degradation. An ortho-nitrobenzylether (o-NBE) moiety is used as a polymerizable cap for poly (ethylene glycol) chains to create a photodegradable macromer. Positive and negative features can be patterned into/onto a hydrogel over a broad range of length scales (∼ 10− ...
