Phytochemistry

Flavanone 8-dimethylallyltransferase in Sophora flavescens cell suspension cultures

H Yamamoto, M Senda, K Inoue

Index: Yamamoto, Hirobumi; Senda, Masayuki; Inoue, Kenichiro Phytochemistry, 2000 , vol. 54, # 7 p. 649 - 655

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Citation Number: 37

Abstract

Dimethylallyl diphosphate: naringenin 8-dimethylallyltransferase (EC 2.5. 1) was characterized. The enzyme was enantiospecific for (−)-(2S)-naringenin and utilized 3, 3- dimethylallyl diphosphate as sole prenyl donor. It required Mg2+ (optimum concentration, 10 mM), and has an optimum pH of 9–10. The apparent Km values for 3, 3-dimethylallyl diphosphate and naringenin were 120 and 36 μM, respectively. The microsomal fraction ...