Formation of γ-Glutamyl Peptides by Glutaminase of Aspergillus oryzae

K Tomita, T Yano, T Kitagata, H Kumagai…

Index: Tomita, Kenji; Yano, Toshihiro; Kitagata, Tatsuichiro; Kumagai, Hidehiko; Tochikura, Tatsurokuro Agricultural and Biological Chemistry, 1989 , vol. 53, # 7 p. 1995 - 1996

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Citation Number: 5

Abstract

In the course of a study on glutaminase of koji mold, we found that the enzyme purified from koji mold, Aspergillus oryzae MA-27-IM, could catalyze the y-glutamyltranspeptidation (GGT) reaction.!) This was the first reported case of a filamentous fungus, though GGT reactions in animals and bacteria have been reported2-S) In this reaction, the y-glutamyl moiety of Ly- glutamyl-p-nitroanilide was transferred to a peptide, glycylglycine, to yield y- ...