Liquid??Chromatography Quantitative Analysis of 20 Amino Acids after Derivatization with FMOC??Cl and Its Application to Different Origin Radix isatidis

W Zhou, XY Zhanga, GL Duana

Index: Zhou, Wei; Zhang, Xiao-Yan; Duan, Geng-Li Journal of the Chinese Chemical Society, 2011 , vol. 58, # 4 p. 509 - 515

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Citation Number: 8

Abstract

Abstract We developed a simple, rapid and reliable method for determination of 20 common amino acids based on derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) and RP-LC/UV, this method was first introduced into quantitative analysis of amino acids. The amino groups of amino acids were trapped with FMOC-Cl to form amino acid-FMOC-Cl adducts which can be suitable for LC-UV. Chromatographic separation was performed on ...