Comparison of substituted 2-nitrophenol degradation by enzyme extracts and intact cells

…, R Stierli, RP Schwarzenbach, J Zeyer

Index: Folsom, Brian R.; Stierli, Ruth; Schwarzenbach, Rene P.; Zeyer, Josef Environmental Science & Technology, 1994 , vol. 28, # 2 p. 306 - 311

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Citation Number: 11

Abstract

The first catabolic pathway enzyme, nitrophenol oxygenase, transforms o-nitrophenol (ONP) to catechol. Thirteen of 16 substituted nitrophenols tested were actively transformed by both enzyme preparations and intact cells yielding a wide range of Km (K,) and Vmax. Individual chemicals in binary mixtures demonstrated competitive inhibition. Chemical and physical characteristics (electron withdrawal, size, and position of substitution on the 2-nitrophenol ...