末端脱氧核苷酸转移酶_MSDS_用途_结构式_CAS号【9027-67-2】

末端脱氧核苷酸转移酶结构式	常用名	末端脱氧核苷酸转移酶	英文名	Terminal Deoxynucleotidyl Transferase
CAS号	9027-67-2	分子量	N/A
密度	N/A	沸点	N/A
分子式	N/A	熔点	N/A
MSDS	美版	闪点	N/A

中文名	末端脱氧核苷酸转移酶
英文名	Terminal Deoxynucleotidyl Transferase
英文别名	更多

描述	末端脱氧核糖核苷酸转移酶(TdT)催化脱氧核糖核苷酸三磷酸酯缩合到DNA链的3'羟基端,并在V(D)J重组期间将N区添加到基因片段连接处。末端脱氧核糖核苷酸转移酶在未成熟、前B、前T淋巴细胞和急性淋巴细胞白血病/淋巴瘤细胞中表达[1]。
相关类别	研究领域 >> 其他信号通路 >> 其他 >> 其他
参考文献	[1]. Boulé JB, et, al. Terminal deoxynucleotidyl transferase indiscriminately incorporates ribonucleotides and deoxyribonucleotides. J Biol Chem. 2001 Aug 17;276(33):31388-93.

储存条件	充氩-20°C密闭，避光，通风干燥处
稳定性	Analysis Note Activity assay: 200 mM potassium cacodylate, pH 7.2, 4 mM MgCl2, 1 mM 2-mercaptoethanol, 1 mM 3H-dATP, 70 μM d(pT)6, 37 °C. Biochem/physiol Actions Bovine terminal transferase (TdT) is a primer-dependent polymerase that catalyzes the addition of deoxynucleotides to the 3′-OH terminus of DNA molecules with the release of inorganic phosphate. TdT reacts preferentially with either single-stranded DNA molecules or double-stranded-DNA with 3′ overhangs, but procedures have been developed to label blunt ends or 3′-recessive ends. In a reaction mixture, the divalent ion (Co2+, Mn2+, Mg2+) will influence purine and pyrimidine polymerization rate. Activities of TdT are also affected by the bases (dATP, dCTP, dGTP and dTTP) present. Each reaction mixture needs to be optimized. The enzyme is commonly used to add complementary homopolymer tails to vector and insert DNA for construction of recombinant DNA in vitro. Features and Benefits • Add homopolymers to vectors, inserts and cDNA for cloning • Label the 3′-end of double- and single-stranded DNA with non-radioactive and radioactive labels • Carry out in vitro mutagenesis by adding single nucleotides to DNA • Use in TUNEL assays with deoxyuridine triphosphate (dUTP) conjugated with a fluorescent chromophore or biotin to label nicked DNA in apoptotic cells Unit Definition One unit will incorporate 1 nanomole of dATP into acid-precipitable material in one hour at 37 °C using d(pT)6 as primer. Physical form Solution in 50 mM potassium phosphate, pH 7.4, 1 mM 2-mercaptoethanol and 50% glycerol (v/v)
更多	1. 性状：冷冻干燥含氯化钾的固体。 2. 密度（g/mL,25℃）：未确定 3. 相对蒸汽密度（g/mL,空气=1）：未确定 4. 熔点（ºC）：未确定 5. 沸点（ºC,常压）：未确定 6. 沸点（ºC,5.2kPa）：未确定 7. 折射率：未确定 8. 闪点（ºC）：未确定 9. 比旋光度（º）：未确定 10. 自燃点或引燃温度（ºC）：未确定 11. 蒸气压（kPa,20ºC）：未确定 12. 饱和蒸气压（kPa,60ºC）：未确定 13. 燃烧热（KJ/mol）：未确定 14. 临界温度（ºC）：未确定 15. 临界压力（KPa）：未确定 16. 油水（辛醇/水）分配系数的对数值：未确定 17. 爆炸上限（%,V/V）：未确定 18. 爆炸下限（%,V/V）：未确定 19. 溶解性：未确定

储存条件

充氩-20°C密闭，避光，通风干燥处

稳定性

Analysis Note Activity assay: 200 mM potassium cacodylate, pH 7.2, 4 mM MgCl2, 1 mM 2-mercaptoethanol, 1 mM 3H-dATP, 70 μM d(pT)6, 37 °C.

Biochem/physiol Actions Bovine terminal transferase (TdT) is a primer-dependent polymerase that catalyzes the addition of deoxynucleotides to the 3′-OH terminus of DNA molecules with the release of inorganic phosphate. TdT reacts preferentially with either single-stranded DNA molecules or double-stranded-DNA with 3′ overhangs, but procedures have been developed to label blunt ends or 3′-recessive ends. In a reaction mixture, the divalent ion (Co2+, Mn2+, Mg2+) will influence purine and pyrimidine polymerization rate. Activities of TdT are also affected by the bases (dATP, dCTP, dGTP and dTTP) present. Each reaction mixture needs to be optimized. The enzyme is commonly used to add complementary homopolymer tails to vector and insert DNA for construction of recombinant DNA in vitro.

Features and Benefits • Add homopolymers to vectors, inserts and cDNA for cloning

• Label the 3′-end of double- and single-stranded DNA with non-radioactive and radioactive labels

• Carry out in vitro mutagenesis by adding single nucleotides to DNA

• Use in TUNEL assays with deoxyuridine triphosphate (dUTP) conjugated with a fluorescent chromophore or biotin to label nicked DNA in apoptotic cells

Unit Definition One unit will incorporate 1 nanomole of dATP into acid-precipitable material in one hour at 37 °C using d(pT)6 as primer.

Physical form Solution in 50 mM potassium phosphate, pH 7.4, 1 mM 2-mercaptoethanol and 50% glycerol (v/v)

更多

1. 性状：冷冻干燥含氯化钾的固体。

2. 密度（g/mL,25℃）：未确定

3. 相对蒸汽密度（g/mL,空气=1）：未确定

4. 熔点（ºC）：未确定

5. 沸点（ºC,常压）：未确定

6. 沸点（ºC,5.2kPa）：未确定

7. 折射率：未确定

8. 闪点（ºC）：未确定

9. 比旋光度（º）：未确定

10. 自燃点或引燃温度（ºC）：未确定

11. 蒸气压（kPa,20ºC）：未确定

12. 饱和蒸气压（kPa,60ºC）：未确定

13. 燃烧热（KJ/mol）：未确定

14. 临界温度（ºC）：未确定

15. 临界压力（KPa）：未确定

16. 油水（辛醇/水）分配系数的对数值：未确定

17. 爆炸上限（%,V/V）：未确定

18. 爆炸下限（%,V/V）：未确定

19. 溶解性：未确定

末端脱氧核苷酸转移酶生态学数据:

通常对水是不危害的，若无政府许可，勿将材料排入周围环境。

末端脱氧核苷酸转移酶毒性英文版

个人防护装备	Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)
危险品运输编码	NONH for all modes of transport

TdT+ T-lymphoblastic populations are increased in Castleman disease, in Castleman disease in association with follicular dendritic cell tumors, and in angioimmunoblastic T-cell lymphoma.

Am. J. Surg. Pathol. 36(11) , 1619-28, (2012)

T-lymphoblastic lymphoma is an aggressive neoplasm requiring prompt clinical treatment. Conversely, indolent T-lymphoblastic proliferation mimics T-lymphoblastic lymphoma but consists of a proliferati...

The pattern of gene expression and gene dose profiles of 6-Mercaptopurine- and 6-Thioguanine-resistant human leukemia cells.

Biochem. Biophys. Res. Commun. 411(1) , 156-61, (2011)

Exposure of MOLT4 human T-cell leukemia cells to 6-Mercaptopurine (6-MP) and 6-Thioguanine (6-TG) resulted in acquired resistance associated with attenuated expression of the genes encoding concentrat...

Polymerase incorporation of pyrene-nucleoside triphosphates.

Bioorg. Med. Chem. Lett. 22(13) , 4428-30, (2012)

Pyrene-deoxynucleoside triphosphates (dPTPs), varying by the positioning of the aromatic system, were synthesized. Their ability to function as substrates for the Klenow fragment of Escherichia coli D...

MFCD00166324

末端脱氧核苷酸转移酶

末端脱氧核苷酸转移酶用途

末端脱氧核苷酸转移酶名称

末端脱氧核苷酸转移酶生物活性

末端脱氧核苷酸转移酶物理化学性质

末端脱氧核苷酸转移酶毒性和生态

末端脱氧核苷酸转移酶安全信息

末端脱氧核苷酸转移酶文献45

末端脱氧核苷酸转移酶英文别名