HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Onchocerca ochengi

External ID: CHEMBL2093846

Protocol: N/A

Comment: Target ChEMBL ID: CHEMBL2093135
ChEMBL Target Name: Onchocerca ochengi
ChEMBL Target Type: ORGANISM - Target is a complete organism
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Data Source: WHO Tropical Disease Research (TDR) Malaria Screening

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	60	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	100	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Onchocerca ochengi

External ID: CHEMBL2093845

Protocol: N/A

Comment: Target ChEMBL ID: CHEMBL2093135
ChEMBL Target Name: Onchocerca ochengi
ChEMBL Target Type: ORGANISM - Target is a complete organism
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Data Source: WHO Tropical Disease Research (TDR) Malaria Screening

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	100	%
Inhibition	=	100	%
Inhibition	=	100	%
Inhibition	=	100	%
Inhibition	=	100	%
Inhibition	=	100	%
Inhibition	=	0	%
Inhibition	=	100	%
Inhibition	=	100	%
Inhibition	=	100	%
Inhibition	=	100	%
Inhibition	=	0	%
Inhibition	=	100	%
Inhibition	=	100	%
Inhibition	=	0	%
Inhibition	=	50	%
Inhibition	=	100	%
Inhibition	=	50	%
Inhibition	=	100	%
Inhibition	=	0	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Trypanosoma cruzi

External ID: CHEMBL2093838

Protocol: N/A

Comment: Target ChEMBL ID: CHEMBL368
ChEMBL Target Name: Trypanosoma cruzi
ChEMBL Target Type: ORGANISM - Target is a complete organism
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Data Source: WHO Tropical Disease Research (TDR) Malaria Screening

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	>	11.76	ug.mL-1
IC50		3.282	ug.mL-1
IC50		3.303	ug.mL-1
IC50	=	0.305	ug.mL-1
IC50	>	8.92	ug.mL-1
IC50	>	12.94	ug.mL-1
IC50	=	4.255	ug.mL-1
IC50	=	5.134	ug.mL-1
IC50	>	13.07	ug.mL-1
IC50	=	2.928	ug.mL-1
IC50	>	10.72	ug.mL-1
IC50	=	0.398	ug.mL-1
IC50	=	0.511	ug.mL-1
IC50	=	0.345	ug.mL-1
IC50	=	0.929	ug.mL-1
IC50	=	5.242	ug.mL-1
IC50	=	5.157	ug.mL-1
IC50		3.089	ug.mL-1
IC50	=	0.575	ug.mL-1
IC50	=	0.442	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Trypanosoma brucei brucei

External ID: CHEMBL2093839

Protocol: N/A

Comment: Target ChEMBL ID: CHEMBL612851
ChEMBL Target Name: Trypanosoma brucei brucei
ChEMBL Target Type: ORGANISM - Target is a complete organism
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Data Source: WHO Tropical Disease Research (TDR) Malaria Screening

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	>	11.76	ug.mL-1
IC50	>	5.735	ug.mL-1
IC50		2.534	ug.mL-1
IC50	=	3.231	ug.mL-1
IC50	>	8.92	ug.mL-1
IC50	>	12.94	ug.mL-1
IC50	=	5.005	ug.mL-1
IC50	>	11.95	ug.mL-1
IC50	>	13.07	ug.mL-1
IC50	=	3.56	ug.mL-1
IC50	=	0.422	ug.mL-1
IC50	=	0.292	ug.mL-1
IC50	=	4.55	ug.mL-1
IC50	=	0.152	ug.mL-1
IC50	=	4.804	ug.mL-1
IC50	>	10.99	ug.mL-1
IC50	=	3.689	ug.mL-1
IC50		3.135	ug.mL-1
IC50	>	12.39	ug.mL-1
IC50	=	4.317	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MRC5

External ID: CHEMBL2093836

Protocol: N/A

Comment: Target ChEMBL ID: CHEMBL614181
ChEMBL Target Name: MRC5
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Data Source: WHO Tropical Disease Research (TDR) Malaria Screening

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	>	11.76	ug.mL-1
IC50		1.813	ug.mL-1
IC50		3.012	ug.mL-1
IC50	=	2.04	ug.mL-1
IC50	>	8.92	ug.mL-1
IC50	>	12.94	ug.mL-1
IC50	>	13.12	ug.mL-1
IC50	>	11.95	ug.mL-1
IC50	>	13.07	ug.mL-1
IC50	>	11	ug.mL-1
IC50	>	10.72	ug.mL-1
IC50	=	1.312	ug.mL-1
IC50	=	5.889	ug.mL-1
IC50	>	9.013	ug.mL-1
IC50	>	14.84	ug.mL-1
IC50	>	10.99	ug.mL-1
IC50	>	9.821	ug.mL-1
IC50		1.955	ug.mL-1
IC50	=	1.527	ug.mL-1
IC50	=	6.19	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：24983 靶标：Huntingtin

External ID: KUHTS-Muma KU-CaM-Htt INH-01

Protocol: 1; Dispense 45 nl compounds (10 mM stocks) using ECHO 555 to Alpha 384 well assay plates. Dispense 45 nl DMSO to control columns 1 and 2 of 384 well plates.
2; Incubate 5 ul of 6XHis-mHTT (Final, 13 nM) with the compounds for 40 mins at room temperature in buffer containing 10 mM Tris.HCl pH 7.4, 1 mM calcium chloride, 150 mM sodium chloride, 0.1% BSA and 20% glycerol.
3; Dispense 5 ul of 6XGST-CaM (Final 13 nM) in buffer A.
4; Incubation; 1 hour (dark at 25C)
5; Dispense 20 ul of Nickel chelate acceptor beads (Final, 20 ugs/ml) and Glutathione donor beads (Final, 30 ugs/ml). Incubate for 2h, room temperature.
6; Detector: Perkin Elmer Enspire, Alphascreen Module (Excitation 680nm/Emission 570nm).

NOTES (numbers refer to Sequence numbers above)
1. Alphascreen bead incubations were performed in green light, TiterTek setting 400rpm.
2. All incubation and addition steps were followed by mixing and centrifugation at 400g,1 min.
3. The percent inhibition for each compound was calculated as follows:
100- [100 *((Test Compound-Median Low Control) / (Median High Control - Median Low Control))]

Where:
Test_Compound is defined as wells containing His mHTT + GST CaM in the presence of test compound

High_Control is defined as wells containing His mHTT + GST CaM and DMSO.

Low_Control is defined as wells containing His mHTT and DMSO.

Comment: All percent inhibition data reported were normalized to high and low controls on a per-plate basis. The results of primary screening data include compounds contributing to assay signal interference, interference with tags binding to Alpha beads, chelators, process related artifacts etc.. The actives were defined as compounds that inhibited Alphascreen reads to greater than 50%.

Activity at 15 uM	Phenotype
-3.1
4.2
-4.8
-0.6
-3.9
-6
75.3	Inhibitor
-10.3
-7
-2
-3.8
17.6
-4.8
-4.1
-7.8
-7.5
15.2
7.8
-2.5
-13.4

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Plasmodium falciparum K1

External ID: CHEMBL2093837

Protocol: N/A

Comment: Target ChEMBL ID: CHEMBL612856
ChEMBL Target Name: Plasmodium falciparum K1
ChEMBL Target Type: ORGANISM - Target is a complete organism
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Data Source: WHO Tropical Disease Research (TDR) Malaria Screening

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	0.24	ug.mL-1
IC50	>	5.735	ug.mL-1
IC50		0.566	ug.mL-1
IC50	=	9.025	ug.mL-1
IC50	>	8.92	ug.mL-1
IC50	>	12.94	ug.mL-1
IC50	>	13.12	ug.mL-1
IC50	>	11.95	ug.mL-1
IC50	>	13.07	ug.mL-1
IC50	>	11	ug.mL-1
IC50	>	10.72	ug.mL-1
IC50	=	8.258	ug.mL-1
IC50	=	10.23	ug.mL-1
IC50	>	9.013	ug.mL-1
IC50	=	0.7798	ug.mL-1
IC50	=	7.351	ug.mL-1
IC50	=	4.264	ug.mL-1
IC50	>	9.912	ug.mL-1
IC50	>	12.39	ug.mL-1
IC50	>	13.34	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Leishmania infantum

External ID: CHEMBL2093840

Protocol: N/A

Comment: Target ChEMBL ID: CHEMBL612848
ChEMBL Target Name: Leishmania infantum
ChEMBL Target Type: ORGANISM - Target is a complete organism
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Data Source: WHO Tropical Disease Research (TDR) Malaria Screening

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	>	11.76	ug.mL-1
IC50	>	5.735	ug.mL-1
IC50	>	9.19	ug.mL-1
IC50	=	3.714	ug.mL-1
IC50	>	8.92	ug.mL-1
IC50	>	12.94	ug.mL-1
IC50	>	13.12	ug.mL-1
IC50	>	11.95	ug.mL-1
IC50	=	4.231	ug.mL-1
IC50	>	11	ug.mL-1
IC50	>	10.72	ug.mL-1
IC50	=	3.737	ug.mL-1
IC50	=	9.575	ug.mL-1
IC50	>	9.013	ug.mL-1
IC50	>	14.84	ug.mL-1
IC50	>	10.99	ug.mL-1
IC50	>	9.821	ug.mL-1
IC50		3.209	ug.mL-1
IC50	=	4.011	ug.mL-1
IC50	=	4.317	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Schistosoma mansoni

External ID: CHEMBL2093841

Protocol: N/A

Comment: Target ChEMBL ID: CHEMBL612893
ChEMBL Target Name: Schistosoma mansoni
ChEMBL Target Type: ORGANISM - Target is a complete organism
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Data Source: WHO Tropical Disease Research (TDR) Malaria Screening

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	25	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%
Inhibition	=	0	%

941908-06-1 靶点实验数据