External ID: MCF7_OneDose

Protocol: NCI-60 Human Tumor Cell Lines Screen was performed using the standard one-dose NCI protocol, which is described in more detail at https://dtp.cancer.gov/discovery_development/nci-60/default.htm.

Compounds with GIPRCNT values below 10 were considered active. Activity score was based on GIPRCNT using the following formula:
max(-GIPRCNT/2 + 50, 0)
Score is 0 for GIPRCNT values of 100 or above, 50 for GIPRCNT values of 0, and 100 for GIPRCNT values of -100.

Comment: These data are a subset of the data from the NCI human tumor cell line screen. Compounds are identified by the NCI National Service Center (NSC) number, which is contained in the
PUBCHEM_EXT_DATASOURCE_REGID field. In the NCI cell line identification system, MCF7 is panel number 5, cell number 1. PANELCODE or PANELNAME identify the NCI-60 cell panel. PANELNBR does NOT identify the NCI-60 panel.

EXPID is the NCI internal tracking number for the experiment identifier, and PREFIX = 'S' for the NSCs submitted through the NCI Chemical Agents repository.

Substance concentration unit is indicated in the data table, using the following abbreviations: M = molar (M), u = micrograms/milliliter (microg/mL), V = volumetric fraction.

M_GIPRCNT represents the mean growth percent for that experiment, and N_GIPRCNT indicates the number of values for that NSC within an experiment, while STDEV_GIPRCNT is the standard deviation. Most NSCs are tested once per experiment, resulting in EXP_COUNT = 1.

External ID: IGROV1_OneDose

Protocol: NCI-60 Human Tumor Cell Lines Screen was performed using the standard one-dose NCI protocol, which is described in more detail at https://dtp.cancer.gov/discovery_development/nci-60/default.htm.

Compounds with GIPRCNT values below 10 were considered active. Activity score was based on GIPRCNT using the following formula:
max(-GIPRCNT/2 + 50, 0)
Score is 0 for GIPRCNT values of 100 or above, 50 for GIPRCNT values of 0, and 100 for GIPRCNT values of -100.

Comment: These data are a subset of the data from the NCI human tumor cell line screen. Compounds are identified by the NCI National Service Center (NSC) number, which is contained in the
PUBCHEM_EXT_DATASOURCE_REGID field. In the NCI cell line identification system, IGROV1 is panel number 6, cell number 10. PANELCODE or PANELNAME identify the NCI-60 cell panel. PANELNBR does NOT identify the NCI-60 panel.

EXPID is the NCI internal tracking number for the experiment identifier, and PREFIX = 'S' for the NSCs submitted through the NCI Chemical Agents repository.

Substance concentration unit is indicated in the data table, using the following abbreviations: M = molar (M), u = micrograms/milliliter (microg/mL), V = volumetric fraction.

M_GIPRCNT represents the mean growth percent for that experiment, and N_GIPRCNT indicates the number of values for that NSC within an experiment, while STDEV_GIPRCNT is the standard deviation. Most NSCs are tested once per experiment, resulting in EXP_COUNT = 1.

External ID: MH0882340 - IMR-90 (cell viability counter screen)

Protocol: IMR-90 assay protocol for automated screening procedures

1 1,000 cells/20 uL IMR-90 cells are seeded per well of a tissue-cultured treated 384-well microtiter assay plate

2 5 uL of each compound dilution is added per well of cells (0-25 uM concentration range).

3 Assay plates are incubated with compound for 48 hours in a 37oC tissue culture incubator.

4 5 uL of Alamar blue is added to each assay plate well.

5 Assay plates are incubated for 4 hours.

6 Data was captured on a fluorescence plate reader(A560/A590).

Comment: PUBCHEM_ACTIVITY_OUTCOME
1 - Substance is considered active when the mean IC50 is < 25uM
2 - Substance is considered inactive when the mean IC50 is > 25 uM
PUBCHEM_ACTIVITY_SCORE
20 - Compounds that were inactive in all triplicate 20-pt dose response assays with a mean IC50 > 25 uM.
40 - Compounds that were active in one of the triplicate 20-pt dose response assays with an IC50 < 25 uM but exhibited an IC50 > or = 25 uM in the other runs.
60 - Compounds that were active in two or three 20-pt dose response assays with a mean IC50 in the 15to 25 uM range
70 - Compounds that were active in two or three 20-pt dose response assays with a mean IC50 in the 10 to 15 uM range
80 - Compounds that were active in two or three 20-pt dose response assays with a mean IC50 in the 5 to 10 uM range
90 - Compounds that were active in two or three 20-pt dose response assays with a mean IC50 in the 0 to 5 uM range