HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Solute carrier organic anion transporter family member 1B1

External ID: CHEMBL3039488

Protocol: N/A

Comment: Journal: Mol Pharmacol
Year: 2013
Volume: 83
Issue: 6
First Page: 1257
Last Page: 1267
DOI: 10.1124/mol.112.084152

Target ChEMBL ID: CHEMBL1697668
ChEMBL Target Name: Solute carrier organic anion transporter family member 1B1
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: H - Homologous protein target assigned
Confidence: Homologous single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	65.85	%
Inhibition	=	63.37	%
Inhibition	=	75.25	%
Inhibition	=	97.38	%
Inhibition	=	105.26	%
Inhibition	=	97.94	%
Inhibition	=	105.32	%
Inhibition	=	99.4	%
Inhibition	=	101.01	%
Inhibition	=	86.27	%
Inhibition	=	80.06	%
Inhibition	=	89.01	%
Inhibition	=	120.91	%
Inhibition	=	102.46	%
Inhibition	=	101.26	%
Inhibition	=	90.74	%
Inhibition	=	113.76	%
Inhibition	=	82.92	%
Inhibition	=	126.2	%
Inhibition	=	116.63	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：24565 靶标：N/A

External ID: ERK5 transcriptional activity-HTS

Protocol: Stable cells plated on a 384-well plate (2500 cells/well) were treated with test compounds at the concentration of 5 muM for 18 hrs. The level of luciferase activity was assayed using a
Luciferase kit (Promega corporation, Madison, WI) and a series of positive and negative control compounds were used as references.

Comment:

Luciferase activity (AU)
104
108
64
28
100
152
176
52
124
44
60
60
32
96
60
144
28
64
84
44

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Solute carrier organic anion transporter family member 1B3

External ID: CHEMBL3039491

Protocol: N/A

Comment: Journal: Mol Pharmacol
Year: 2013
Volume: 83
Issue: 6
First Page: 1257
Last Page: 1267
DOI: 10.1124/mol.112.084152

Target ChEMBL ID: CHEMBL1743121
ChEMBL Target Name: Solute carrier organic anion transporter family member 1B3
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: H - Homologous protein target assigned
Confidence: Homologous single protein target assigned

Standard Type	Standard Relation	Standard Value	Standard Units	Data Validity Comment
Inhibition	=	89.51	%
Inhibition	=	81.71	%
Inhibition	=	65.47	%
Inhibition	=	50.32	%
Inhibition	=	112.43	%
Inhibition	=	99.94	%
Inhibition	=	101.75	%
Inhibition	=	98.72	%
Inhibition	=	104.08	%
Inhibition	=	114.2	%
Inhibition	=	97.5	%
Inhibition	=	120.61	%
Inhibition	=	45.56	%
Inhibition	=	105.72	%
Inhibition	=	107.28	%
Inhibition	=	93.09	%
Inhibition	=	84.51	%
Inhibition	=	155.79	%	Outside typical range
Inhibition	=	97.26	%
Inhibition	=	72.91	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HL-60

External ID: CHEMBL4134394

Protocol: N/A

Comment: Journal: Eur J Med Chem
Year: 2017
Volume: 140
First Page: 305
Last Page: 320
DOI: 10.1016/j.ejmech.2017.08.011

Target ChEMBL ID: CHEMBL383
ChEMBL Target Name: HL-60
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Not Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：11827 靶标：Phosphogluconate dehydrogenase

External ID: 6PGD_Inhibitor_Screening_2015_09

Protocol: The screening assay was performed in a 96-well reading plate, the reaction of each well is 200ul which contains:

50 mM Tris (pH 8.1),
0.2 mM 6-phosphogluconate,
1 mM MgCl2,
0.1 mM NADP+,
0.5 ug recombinant human 6PGD protein,
and 10 uM compound candidate.

The increase in absorbance at 341 nm was measured using a SpectraMax Plus spectrophotometer (Molecular Devices)

Comment: Please note:
This is primary screening data, and for further confirmation, compound that shows equal or over 20% inhibition of 6PGD enzyme activity in this assay is considered as a potential inhibitor.

Percentage of inhibition	BIOACTIVITY_PHENOTYPE
-7.36402
-2.25941
0.502092
-6.10879
1.841
-10.795
-25.3556
-10.6276
-7.61506
10.0833
-2.91667
2.66667
-18.75
-18.3333
-19.4167
-24.5
3.16667
13.5
5.47112
10.9422

80-99-9 靶点实验数据