HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885703

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	0.6	%
Inhibition	=	38	%
Inhibition	=	-9.3	%
Inhibition	=	3.3	%
Inhibition	=	50.5	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885704

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	5.5	%
Inhibition	=	19.6	%
Inhibition	=	50.2	%
Inhibition	=	29.6	%
Inhibition	=	30.6	%
Inhibition	=	79.8	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885705

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	35.1	%
Inhibition	=	53.7	%
Inhibition	=	58	%
Inhibition	=	49.2	%
Inhibition	=	64.9	%
Inhibition	=	87.8	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885710

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	12.5	%
Inhibition	=	0.7	%
Inhibition	=	10.9	%
Inhibition	=	3.8	%
Inhibition	=	5.5	%
Inhibition	=	10.3	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885711

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	95.9	%
Inhibition	=	78.3	%
Inhibition	=	96.4	%
Inhibition	=	95.8	%
Inhibition	=	43.7	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885712

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	3.8	%
Inhibition	=	97.5	%
Inhibition	=	96.5	%
Inhibition	=	97.9	%
Inhibition	=	96.9	%
Inhibition	=	48.7	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885713

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	9.3	%
Inhibition	=	97.3	%
Inhibition	=	97.7	%
Inhibition	=	98.1	%
Inhibition	=	96.4	%
Inhibition	=	50	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885706

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	43.2	%
Inhibition	=	51.6	%
Inhibition	=	57.9	%
Inhibition	=	67.6	%
Inhibition	=	54.6	%
Inhibition	=	89.2	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885707

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	9.4	%
Inhibition	=	6.3	%
Inhibition	=	-1.9	%
Inhibition	=	-2	%
Inhibition	=	4.7	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885708

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	-6.2	%
Inhibition	=	9.9	%
Inhibition	=	8.6	%
Inhibition	=	0.8	%
Inhibition	=	0.8	%
Inhibition	=	12.6	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885709

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	-1.2	%
Inhibition	=	9.5	%
Inhibition	=	10.7	%
Inhibition	=	2	%
Inhibition	=	2.4	%
Inhibition	=	12.2	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885718

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	22.5	%
Inhibition	=	97.8	%
Inhibition	=	98.9	%
Inhibition	=	98.6	%
Inhibition	=	98.5	%
Inhibition	=	79.9	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885719

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	16.2	%
Inhibition	=	10.5	%
Inhibition	=	5	%
Inhibition	=	17.2	%
Inhibition	=	-0.5	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885720

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	2.8	%
Inhibition	=	17.1	%
Inhibition	=	-0.9	%
Inhibition	=	7	%
Inhibition	=	21.8	%
Inhibition	=	10.8	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885721

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	6.1	%
Inhibition	=	23	%
Inhibition	=	-1.8	%
Inhibition	=	5.9	%
Inhibition	=	24.4	%
Inhibition	=	12.4	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885714

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	25.3	%
Inhibition	=	96.9	%
Inhibition	=	97.3	%
Inhibition	=	98	%
Inhibition	=	96	%
Inhibition	=	50.3	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885715

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	98.6	%
Inhibition	=	98.2	%
Inhibition	=	98.7	%
Inhibition	=	98.3	%
Inhibition	=	65.6	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885716

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	-0.9	%
Inhibition	=	98.5	%
Inhibition	=	98.7	%
Inhibition	=	98.7	%
Inhibition	=	98.7	%
Inhibition	=	79.8	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885717

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	1.7	%
Inhibition	=	98.4	%
Inhibition	=	98.8	%
Inhibition	=	98.9	%
Inhibition	=	98.3	%
Inhibition	=	81.4	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885726

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	17.6	%
Inhibition	=	40.4	%
Inhibition	=	40.4	%
Inhibition	=	83.2	%
Inhibition	=	53.6	%
Inhibition	=	70	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：N/A

External ID: CHEMBL4191765

Protocol: N/A

Comment: Journal: J Nat Prod
Year: 2017
Volume: 80
Issue: 7
First Page: 2060
Last Page: 2066
DOI: 10.1021/acs.jnatprod.7b00255

Standard Type	Standard Units	Activity Comment
Inhibition	%	Not Active
Inhibition	%	Not Active
Inhibition	%	Not Active
Inhibition	%	Not Active
Inhibition	%	Not Active
Inhibition	%	Not Active
Inhibition	%	Not Active
Inhibition	%	Not Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885727

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	97.4	%
Inhibition	=	95.3	%
Inhibition	=	97.8	%
Inhibition	=	97.3	%
Inhibition	=	59.8	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885728

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	1.5	%
Inhibition	=	95.3	%
Inhibition	=	98.5	%
Inhibition	=	97.9	%
Inhibition	=	97.1	%
Inhibition	=	87	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885729

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	6	%
Inhibition	=	91.4	%
Inhibition	=	98.5	%
Inhibition	=	98.2	%
Inhibition	=	94.9	%
Inhibition	=	87.6	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885722

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	17.3	%
Inhibition	=	26.8	%
Inhibition	=	17	%
Inhibition	=	13.7	%
Inhibition	=	29.2	%
Inhibition	=	10.3	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885723

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	35.2	%
Inhibition	=	35.2	%
Inhibition	=	54	%
Inhibition	=	52.2	%
Inhibition	=	46.2	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885724

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	2	%
Inhibition	=	44.2	%
Inhibition	=	44.2	%
Inhibition	=	63	%
Inhibition	=	50.6	%
Inhibition	=	64.4	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885725

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	5.1	%
Inhibition	=	51	%
Inhibition	=	51	%
Inhibition	=	67.7	%
Inhibition	=	47.6	%
Inhibition	=	70.9	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HL-60

External ID: CHEMBL885734

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL383
ChEMBL Target Name: HL-60
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Activity	=	11.2	%
Activity	=	22.2	%
Activity	=	8.4	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HL-60

External ID: CHEMBL885737

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL383
ChEMBL Target Name: HL-60
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Activity	=	8.3	10^5/ml
Activity	=	2.1	10^5/ml
Activity	=	4.5	10^5/ml

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885730

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Inhibition	=	17.2	%
Inhibition	=	93.4	%
Inhibition	=	98.5	%
Inhibition	=	98.3	%
Inhibition	=	95	%
Inhibition	=	87.7	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885731

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Active
Activity	Active
Activity	Active
Activity	Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-1080

External ID: CHEMBL885732

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL614580
ChEMBL Target Name: HT-1080
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Active
Activity	Active
Activity	Active
Activity	Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：15621 靶标：G protein-activated inward rectifier potassium channel 2

External ID: VANDERBILT_HTS_GIRK2_MPD

Protocol: To screen for modulators of the G protein-gated inwardly-rectifying potassium channel subunit 2 (GIRK2) homomeric channels, HEK293 cells were engineered to overexpress GIRK2. These cells were screened using a high-throughput thallium-flux assay in a 384-well format. High-throughput thallium flux assays are based on three principles: (1) extracellular thallium can permeate through potassium channels into cells, (2) the fluorescent dye Thallos enters and stays in the cytoplasm of HEK293 cells, and (3) the fluorescence of Thallos increases dramatically when it interacts with thallium ions. Compounds that modulate the activity of an overexpressed channel, in this case GIRK2, are identified by the change in fluorescence that results from changes in thallium influx into cells through the overexpressed channel.

The screening protocol was conducted as follows. One hour prior to screening, cells were loaded with 1.5 micromolar (muM) Thallos in assay buffer (1x Hank's Balanced Salt Solution and 20 millimolar (mM) HEPES). The liquid handling and imaging was conducted using a kinetic-imaging plate reader, Panoptic (WaveFront Biosciences). Before screening, Thallos-containing buffer was removed from cells and replaced with 20 microliters (muL) per well of assay buffer. Compounds were dissolved in assay buffer at 20 muM. After 8 seconds of imaging, 20 muL of compound solution was added per well and allowed to incubate for 150 seconds. Next, 10 muL of a solution containing 2.0 mM thallium in Base Buffer (125 mM NaHCO3, 1 mM MgSO4, 1.8 mM CaSO4, 5mM Glucose, and 20 mM HEPES) was added to the wells. 30 seconds later, 12 muL of base buffer containing 2.0 mM thallium and 50 mM 2-methyl-2,4-pentanediol (MPD), a partially-activating concentration, was added to each well. MPD has is a previously-described GIRK2 channel activator. Data was collected for 60 seconds after this final addition. The positive control for this screen was 30 muM ivermectin, a natural product previously identified as a GIRK2 activator as part of a small-scale pilot screen. Microsoft Excel was utilized for data analysis. Data were normalized, F/Fo, on a well-to-well basis to account for differences in cell number. Compound activity was analyzed by comparing the sums of control-subtracted amplitudes of fluorescence intensity at 20 seconds after each thallium addition. The top 0.2% compounds corresponding to wells that demonstrated the largest increases in fluorescence were selected as "hits" for counter screening, as described below.

All 63,228 compounds screened using the method described above are listed in column OUTCOME_SCREEN_HITS. The 133 compounds selected as hits based on the above criteria are labelled with "100" in the ACTIVITY column. Fluorescent compounds were included in the inactive compounds and marked with "0" in the ACTIVITY column.

The 133 active compounds from column OUTCOME_SCREEN_HITS were tested for non-specific activity in untransfected HEK293 cells using assay conditions otherwise identical to the primary screen. Column OUTCOME_HEK_COUNTERSCREEN lists the 23 compounds that displayed an increase in fluorescence upon thallium addition with "100" in the ACTIVITY column.

The 110 compounds which were identified as hits in the primary screen but inactive when tested in untransfected HEK293 cells were tested for activity in GIRK2-overexpressing HEK293 cells that did not express NPY4 receptor. Column OUTCOME_GIRK2_COUNTERSCREEN lists the 44 compound that displayed an increase in fluorescence upon thallium addition with "100" in the ACTIVITY column.

The 44 active compounds from column OUTCOME_GIRK2_COUNTERSCREEN were tested at various concentrations to determine if compound activity was concentration dependent. Column OUTCOME_GIRK2_DOSE_RESPONSE provides the efficacy of these compounds; activity of all compounds at the provided concentrations was normalized to the activity of VU0537695 at 25 muM, which was the highest efficacy observed during this screening. For each compound concentration listed, the efficacy of that compound provided refers to the normalized, control-subtracted fluorescence intensity at 15 seconds after the addition of thallium. The 28 compounds that demonstrated >5% efficacy and concentration-dependent activity were labelled as "100" in the ACTIVITY column.

Finally, column PUBCHEM_ACTIVITY_OUTCOME combines all of the information from the above counterscreens together, indicating the 28 hits from this GIRK2 screen.

Comment:

OUTCOME_SCREEN_HITS	OUTCOME_HEK_COUNTERSCREEN	OUTCOME_GIRK2_COUNTERSCREEN	OUTCOME_GIRK2_DOSE_RESPONSE	% Efficacy @ 25uM	% Efficacy @ 8.3uM	% Efficacy @ 2.8uM	% Efficacy @ 0.94uM	% Efficacy @ 0.15uM	% Efficacy @ 0.024uM
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE
INACTIVE

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HL-60

External ID: CHEMBL885733

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL383
ChEMBL Target Name: HL-60
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Activity	=	4.1	%
Activity	=	12.5	%
Activity	=	5.9	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：U-937

External ID: CHEMBL885742

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL612794
ChEMBL Target Name: U-937
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units
100	IC50	>	100000	nM
100	IC50	>	100000	nM
28	IC50	=	28000	nM
100	IC50	>	100000	nM
100	IC50	>	100000	nM
44	IC50	=	44000	nM
100	IC50	>	100000	nM
16	IC50	=	16000	nM
23	IC50	=	23000	nM
0.07	IC50	=	70	nM
48	IC50	=	48000	nM
100	IC50	>	100000	nM
85	IC50	=	85000	nM
72	IC50	=	72000	nM
25	IC50	=	25000	nM
100	IC50	>	100000	nM
35	IC50	=	35000	nM
32	IC50	=	32000	nM
20	IC50	=	20000	nM
100	IC50	>	100000	nM

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：U-937

External ID: CHEMBL885743

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL612794
ChEMBL Target Name: U-937
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units
100	IC50	>	100000	nM
100	IC50	>	100000	nM
20	IC50	=	20000	nM
100	IC50	>	100000	nM
100	IC50	>	100000	nM
45	IC50	=	45000	nM
100	IC50	>	100000	nM
5.8	IC50	=	5800	nM
7.3	IC50	=	7300	nM
0.09	IC50	=	90	nM
43	IC50	=	43000	nM
100	IC50	>	100000	nM
74	IC50	=	74000	nM
48	IC50	=	48000	nM
21	IC50	=	21000	nM
100	IC50	>	100000	nM
32	IC50	=	32000	nM
28	IC50	=	28000	nM
13	IC50	=	13000	nM
100	IC50	>	100000	nM

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Cavia porcellus

External ID: CHEMBL684375

Protocol: N/A

Comment: Journal: J. Med. Chem.
Year: 1980
Volume: 23
Issue: 9
First Page: 1022
Last Page: 1026
DOI: 10.1021/jm00183a011

Target ChEMBL ID: CHEMBL369
ChEMBL Target Name: Cavia porcellus
ChEMBL Target Type: ORGANISM - Target is a complete organism
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	0.0155	ug.mL-1
IC50	=	0.042	ug.mL-1
IC50	=	0.0238	ug.mL-1
IC50	=	0.062	ug.mL-1
IC50	=	0.0247	ug.mL-1
IC50	=	0.2	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HEp-2

External ID: CHEMBL5389761

Protocol: N/A

Comment: Journal: J Nat Prod
Year: 2024
Volume: 87
Issue: 4
First Page: 1003
Last Page: 1012
DOI: 10.1021/acs.jnatprod.3c01288

Target ChEMBL ID: CHEMBL614735
ChEMBL Target Name: HEp-2
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HL-60

External ID: CHEMBL885738

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL383
ChEMBL Target Name: HL-60
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Activity	=	5.3	10^5/ml
Activity	=	0.3	10^5/ml
Activity	=	1	10^5/ml

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：U-937

External ID: CHEMBL885741

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: Bioorg. Med. Chem.
Year: 2007
Volume: 15
Issue: 3
First Page: 1539
Last Page: 1546
DOI: 10.1016/j.bmc.2006.09.024

Target ChEMBL ID: CHEMBL612794
ChEMBL Target Name: U-937
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units
100	IC50	>	100000	nM
100	IC50	>	100000	nM
36	IC50	=	36000	nM
100	IC50	>	100000	nM
100	IC50	>	100000	nM
50	IC50	=	50000	nM
100	IC50	>	100000	nM
42	IC50	=	42000	nM
30	IC50	=	30000	nM
3.6	IC50	=	3600	nM
49	IC50	=	49000	nM
73	IC50	=	73000	nM
68	IC50	=	68000	nM
100	IC50	>	100000	nM
46	IC50	=	46000	nM
100	IC50	>	100000	nM
28	IC50	=	28000	nM
35	IC50	=	35000	nM
26	IC50	=	26000	nM
100	IC50	>	100000	nM

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：N/A

External ID: CHEMBL5389756

Protocol: N/A

Comment: Journal: J Nat Prod
Year: 2024
Volume: 87
Issue: 4
First Page: 1003
Last Page: 1012
DOI: 10.1021/acs.jnatprod.3c01288

Standard Type	Standard Relation	Standard Value	Activity Comment
Ratio CC50/IC50	<	2.5
Ratio CC50/IC50			Not Determined
Ratio CC50/IC50	=	61.6
Ratio CC50/IC50	=	15.6
Ratio CC50/IC50	=	1
Ratio CC50/IC50	>	9.4
Ratio CC50/IC50	=	11.4
Ratio CC50/IC50	=	6.5
Ratio CC50/IC50	=	28

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HEp-2

External ID: CHEMBL5389755

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: J Nat Prod
Year: 2024
Volume: 87
Issue: 4
First Page: 1003
Last Page: 1012
DOI: 10.1021/acs.jnatprod.3c01288

Target ChEMBL ID: CHEMBL614735
ChEMBL Target Name: HEp-2
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units
12.5	CC50	=	12500	nM
100	CC50	>	100000	nM
100	CC50	>	100000	nM
91	CC50	=	91000	nM
65	CC50	=	65000	nM
100	CC50	>	100000	nM
100	CC50	>	100000	nM
50	CC50	=	50000	nM
100	CC50	>	100000	nM

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Human orthopneumovirus

External ID: CHEMBL5389754

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: J Nat Prod
Year: 2024
Volume: 87
Issue: 4
First Page: 1003
Last Page: 1012
DOI: 10.1021/acs.jnatprod.3c01288

Target ChEMBL ID: CHEMBL613127
ChEMBL Target Name: Human orthopneumovirus
ChEMBL Target Type: ORGANISM - Target is a complete organism
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units
0.8	IC50	=	800	nM
100	IC50	>	100000	nM
10.6	IC50	=	10600	nM
8	IC50	=	8000	nM
10	IC50	=	10000	nM
100	IC50	>	100000	nM
53	IC50	=	53000	nM
1.4	IC50	=	1400	nM
100	IC50	>	100000	nM

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：N/A

External ID: CHEMBL2411673

Protocol: N/A

Comment: Journal: J. Nat. Prod.
Year: 2013
Volume: 76
Issue: 8
First Page: 1498
Last Page: 1504
DOI: 10.1021/np400567c

Standard Type	Standard Relation	Standard Value
Ratio	=	0.9
Ratio	=	35.3
Ratio	=	0.6
Ratio	=	23.9

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：20881 靶标：interleukin-1 receptor-like 1 isoform [homo sapiens]

External ID: ST2_IL33_Inhibitors_Primary_Screening_77700

Protocol: High-throughput screening was performed at Indiana University screening facility (Indiana University, Indianapolis, IN).

In summary, a mixture of ST2 and IL-33 was prepared by adding 0.30 microL of 14.1 microM recombinant human ST2-Fc Chimera and 0.30 microL of 55.25 biotinylated recombinant human IL-33 to 1.4 mL of assay buffer. In the HTS, each well of a 384-well Proxi-plate was first blocked by 100 microL of assay buffer for 1 h at room temperature. After removing the blocking buffer, 20 microL of ST2/IL-33 mixture was pipetted into each well and incubated at room temperature for 1 h followed by addition of compounds at 17 microM (0.8 microL) to each well and incubated at room temperature for 1 h. Ten microliters of 60 microg/mL anti-6xHis-conjugated AlphaLISA acceptor beads were added to each well and incubated at room temperature for 1 h before addition of 10 microL of 60 microg/mL of streptavidin-labeled AlphaLISA donor beads for incubation at room temperature for 30 min. Incubation of acceptor and receptor beads was conducted in darkness. The well with 3 nM ST2 alone was used as a negative control, whereas the ST2/IL-33 mixture with a human ST2 antibody added at 0.45 ng/microL was used as the positive control. Plates were read using the Envision Plate Reader. The buffer used in the assay contains 14.37 mL PBS, 30 microL Tween 20, and 600 microL of 5% bovine serum albumin in PBS.

Comment: PubChem active indicates >= 30% inhibition of ST2/IL-33 at 17 uM of the compound. Inconclusive: >=10% and < 30% inhibition.

% Inhibition
98
97
97
97
97
97
96
96
96
96
95
95
94
94
93
92
92
89
88
85

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：697

External ID: CHEMBL2411677

Protocol: N/A

Comment: Journal: J. Nat. Prod.
Year: 2013
Volume: 76
Issue: 8
First Page: 1498
Last Page: 1504
DOI: 10.1021/np400567c

Target ChEMBL ID: CHEMBL2366253
ChEMBL Target Name: 697
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
Activity	=	24	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：697

External ID: CHEMBL2411676

Protocol: N/A

Comment: Journal: J. Nat. Prod.
Year: 2013
Volume: 76
Issue: 8
First Page: 1498
Last Page: 1504
DOI: 10.1021/np400567c

Target ChEMBL ID: CHEMBL2366253
ChEMBL Target Name: 697
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Activity Comment
Activity	Active

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：N/A

External ID: CHEMBL2411675

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: J. Nat. Prod.
Year: 2013
Volume: 76
Issue: 8
First Page: 1498
Last Page: 1504
DOI: 10.1021/np400567c

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units
0.8	CD	=	0.8	uM
0.2	CD	=	0.2	uM
10	CD	>	10	uM
3.8	CD	=	3.8	uM

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：NON-PROTEIN TARGET

External ID: CHEMBL2411674

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: J. Nat. Prod.
Year: 2013
Volume: 76
Issue: 8
First Page: 1498
Last Page: 1504
DOI: 10.1021/np400567c

Target ChEMBL ID: CHEMBL3879801
ChEMBL Target Name: NON-PROTEIN TARGET
ChEMBL Target Type: NON-MOLECULAR - Target has not been defined at a molecular level, only the non-molecular entity which is affected (e.g., organism, cell line etc)
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units
18.1	IC50	=	18100	nM
63.9	IC50	=	63900	nM
12.1	IC50	=	12100	nM
37.6	IC50	=	37600	nM

76-80-2 靶点实验数据