HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Dihydrofolate reductase

External ID: CHEMBL667316

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: J. Med. Chem.
Year: 1982
Volume: 25
Issue: 2
First Page: 182
Last Page: 187
DOI: 10.1021/jm00344a018

Target ChEMBL ID: CHEMBL4564
ChEMBL Target Name: Dihydrofolate reductase
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units	Data Validity Comment
3.5E-6	Ki	=	0.0035	nM	Outside typical range
0.1701	Ki	=	170.1	nM
3.7E-6	Ki	=	0.0037	nM	Outside typical range
2.7E-6	Ki	=	0.0027	nM	Outside typical range
0.46	Ki	=	460	nM

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：NON-PROTEIN TARGET

External ID: CHEMBL849762

Protocol: N/A

Comment: Journal: J. Med. Chem.
Year: 1982
Volume: 25
Issue: 2
First Page: 182
Last Page: 187
DOI: 10.1021/jm00344a018

Target ChEMBL ID: CHEMBL3879801
ChEMBL Target Name: NON-PROTEIN TARGET
ChEMBL Target Type: NON-MOLECULAR - Target has not been defined at a molecular level, only the non-molecular entity which is affected (e.g., organism, cell line etc)
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value
MTX ratio	=	88
MTX ratio	=	11
MTX ratio	=	23
MTX ratio	=	2.5
MTX ratio	=	370
MTX ratio	=	1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：L1210

External ID: CHEMBL859422

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: J. Med. Chem.
Year: 1982
Volume: 25
Issue: 2
First Page: 182
Last Page: 187
DOI: 10.1021/jm00344a018

Target ChEMBL ID: CHEMBL386
ChEMBL Target Name: L1210
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units
16.9	Km	=	16900	nM
3.9	Km	=	3900	nM
9.4	Km	=	9400	nM
48.4	Km	=	48400	nM

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HEp-2

External ID: CHEMBL687049

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: J Med Chem
Year: 1982
Volume: 25
Issue: 2
First Page: 182
Last Page: 187
DOI: 10.1021/jm00344a018

Target ChEMBL ID: CHEMBL614735
ChEMBL Target Name: HEp-2
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

PubChem Standard Value	Standard Type	Standard Relation	Standard Value	Standard Units
0.055	ED50	=	0.055	uM
0.18	ED50	=	0.18	uM
0.0052	ED50	=	0.0052	uM
0.028	ED50	=	0.028	uM
0.61	ED50	=	0.61	uM

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：L1210

External ID: CHEMBL707760

Protocol: N/A

Comment: Journal: J. Med. Chem.
Year: 1982
Volume: 25
Issue: 2
First Page: 182
Last Page: 187
DOI: 10.1021/jm00344a018

Target ChEMBL ID: CHEMBL386
ChEMBL Target Name: L1210
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
ILS	=	74	%

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：L1210

External ID: CHEMBL701864

Protocol: N/A

Comment: Journal: J Med Chem
Year: 1982
Volume: 25
Issue: 2
First Page: 182
Last Page: 187
DOI: 10.1021/jm00344a018

Target ChEMBL ID: CHEMBL386
ChEMBL Target Name: L1210
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
k	=	0.19	M-1 min-1
k	=	0.21	M-1 min-1
k	=	0.26	M-1 min-1
k	=	2.1	M-1 min-1
k	=	0.23	M-1 min-1

71074-46-9 靶点实验数据