HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Integrin alpha-V

External ID: CHEMBL913667

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem. Lett.
Year: 2006
Volume: 16
Issue: 24
First Page: 6178
Last Page: 6180
DOI: 10.1016/j.bmcl.2006.09.042

Target ChEMBL ID: CHEMBL1907598
ChEMBL Target Name: Integrin alpha-V/beta-3
ChEMBL Target Type: PROTEIN COMPLEX - Target is a defined protein complex, consisting of multiple subunits
Relationship Type: D - Direct protein target assigned
Confidence: Direct protein complex subunits assigned

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	5.97	ug.mL-1
IC50	=	2.8E-4	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：A549

External ID: CHEMBL913668

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem. Lett.
Year: 2006
Volume: 16
Issue: 24
First Page: 6178
Last Page: 6180
DOI: 10.1016/j.bmcl.2006.09.042

Target ChEMBL ID: CHEMBL392
ChEMBL Target Name: A549
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：N/A

External ID: CHEMBL913669

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem. Lett.
Year: 2006
Volume: 16
Issue: 24
First Page: 6178
Last Page: 6180
DOI: 10.1016/j.bmcl.2006.09.042

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：N/A

External ID: CHEMBL913670

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem. Lett.
Year: 2006
Volume: 16
Issue: 24
First Page: 6178
Last Page: 6180
DOI: 10.1016/j.bmcl.2006.09.042

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MCF7

External ID: CHEMBL913675

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem. Lett.
Year: 2006
Volume: 16
Issue: 24
First Page: 6178
Last Page: 6180
DOI: 10.1016/j.bmcl.2006.09.042

Target ChEMBL ID: CHEMBL387
ChEMBL Target Name: MCF7
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：B16

External ID: CHEMBL913676

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem. Lett.
Year: 2006
Volume: 16
Issue: 24
First Page: 6178
Last Page: 6180
DOI: 10.1016/j.bmcl.2006.09.042

Target ChEMBL ID: CHEMBL381
ChEMBL Target Name: B16
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：20881 靶标：interleukin-1 receptor-like 1 isoform [homo sapiens]

External ID: ST2_IL33_Inhibitors_Primary_Screening_77700

Protocol: High-throughput screening was performed at Indiana University screening facility (Indiana University, Indianapolis, IN).

In summary, a mixture of ST2 and IL-33 was prepared by adding 0.30 microL of 14.1 microM recombinant human ST2-Fc Chimera and 0.30 microL of 55.25 biotinylated recombinant human IL-33 to 1.4 mL of assay buffer. In the HTS, each well of a 384-well Proxi-plate was first blocked by 100 microL of assay buffer for 1 h at room temperature. After removing the blocking buffer, 20 microL of ST2/IL-33 mixture was pipetted into each well and incubated at room temperature for 1 h followed by addition of compounds at 17 microM (0.8 microL) to each well and incubated at room temperature for 1 h. Ten microliters of 60 microg/mL anti-6xHis-conjugated AlphaLISA acceptor beads were added to each well and incubated at room temperature for 1 h before addition of 10 microL of 60 microg/mL of streptavidin-labeled AlphaLISA donor beads for incubation at room temperature for 30 min. Incubation of acceptor and receptor beads was conducted in darkness. The well with 3 nM ST2 alone was used as a negative control, whereas the ST2/IL-33 mixture with a human ST2 antibody added at 0.45 ng/microL was used as the positive control. Plates were read using the Envision Plate Reader. The buffer used in the assay contains 14.37 mL PBS, 30 microL Tween 20, and 600 microL of 5% bovine serum albumin in PBS.

Comment: PubChem active indicates >= 30% inhibition of ST2/IL-33 at 17 uM of the compound. Inconclusive: >=10% and < 30% inhibition.

% Inhibition
98
97
97
97
97
97
96
96
96
96
95
95
94
94
93
92
92
89
88
85

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：Entamoeba histolytica

External ID: CHEMBL3058937

Protocol: N/A

Comment: Journal: Med Chem Res
Year: 2012
Volume: 21
Issue: 9
First Page: 2501
Last Page: 2516
DOI: 10.1007/s00044-011-9767-1

Target ChEMBL ID: CHEMBL612853
ChEMBL Target Name: Entamoeba histolytica
ChEMBL Target Type: ORGANISM - Target is a complete organism
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units	Data Validity Comment
IC50	=	12.7	ug.mL-1
IC50	=	114.3	ug.mL-1	Outside typical range
IC50	=	92.24	ug.mL-1	Outside typical range
IC50	=	81	ug.mL-1	Outside typical range
IC50	=	189.01	ug.mL-1	Outside typical range
IC50	=	7.93	ug.mL-1
IC50	=	17.8	ug.mL-1
IC50	=	12.7	ug.mL-1
IC50	=	6.89	ug.mL-1
IC50	=	0.00777	ug.mL-1
IC50	=	22.6	ug.mL-1
IC50	=	115.21	ug.mL-1	Outside typical range
IC50	=	158.67	ug.mL-1	Outside typical range
IC50	=	70.3	ug.mL-1	Outside typical range
IC50	=	0.306	ug.mL-1
IC50	=	16.6	ug.mL-1
IC50	=	23	ug.mL-1
IC50	=	14.8	ug.mL-1
IC50	=	0.14	ug.mL-1
IC50	=	105.3	ug.mL-1	Outside typical range

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：DU-145

External ID: CHEMBL913671

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem. Lett.
Year: 2006
Volume: 16
Issue: 24
First Page: 6178
Last Page: 6180
DOI: 10.1016/j.bmcl.2006.09.042

Target ChEMBL ID: CHEMBL613508
ChEMBL Target Name: DU-145
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：MDA-MB-231

External ID: CHEMBL913672

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem. Lett.
Year: 2006
Volume: 16
Issue: 24
First Page: 6178
Last Page: 6180
DOI: 10.1016/j.bmcl.2006.09.042

Target ChEMBL ID: CHEMBL400
ChEMBL Target Name: MDA-MB-231
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：HT-29

External ID: CHEMBL913673

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem. Lett.
Year: 2006
Volume: 16
Issue: 24
First Page: 6178
Last Page: 6180
DOI: 10.1016/j.bmcl.2006.09.042

Target ChEMBL ID: CHEMBL384
ChEMBL Target Name: HT-29
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1

HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_DryPowder_Activity_Set16

来源：ChEMBL 靶标：N/A

External ID: CHEMBL913674

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem. Lett.
Year: 2006
Volume: 16
Issue: 24
First Page: 6178
Last Page: 6180
DOI: 10.1016/j.bmcl.2006.09.042

Standard Type	Standard Relation	Standard Value	Standard Units
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1
IC50	=	1.6	ug.mL-1

618-67-7 靶点实验数据