External ID: CHEMBL2444904

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: Bioorg. Med. Chem.
Year: 2013
Volume: 21
Issue: 22
First Page: 7107
Last Page: 7117
DOI: 10.1016/j.bmc.2013.09.010

Target ChEMBL ID: CHEMBL2073663
ChEMBL Target Name: Monocarboxylate transporter 4
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

External ID: TELO_02

Protocol: HCT116 colorectal cancer cells are infected with Ad-hTR-mut and cytotoxicity is assessed 5-days post infection, measured by standard MTT assay. Ad-hTR-mut constructed using the Adeasy system (Agilent) and purified and quantified using Adeno-x purification and rapid titre kits (Clontech). Cells are seeded at 10000 cells per well and infected with 500pfu/cell in 96 well plates (Costar) then incubated for 48h prior to addition of compounds. Compounds were screened at 10uM in duplicate 2 days post-infection and MTT assay performed 3 days later at 5 days post-infection. A negative control adenovirus was included on every plate as a control for non-specific viral toxicity and all wells were expressed relative to untreated cells. Absorbance at 570nm was determined using a Molecular Devices spectramax plus instrument and analysed using SoftMax Pro software. Hits were taken to be compounds giving greater than 2.5-fold increase in absorbance indicating increased cell survival.

Comment: Target cell viability at time of assay was 30-50% after infection with this stock of Ad-hTR-mut. Compounds giving 2.5-fold increase in survival relative to this condition were taken to be hits.

External ID: CHEMBL2443718

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2013
Volume: 21
Issue: 22
First Page: 7107
Last Page: 7117
DOI: 10.1016/j.bmc.2013.09.010

Target ChEMBL ID: CHEMBL2073663
ChEMBL Target Name: Monocarboxylate transporter 4
ChEMBL Target Type: SINGLE PROTEIN - Target is a single protein chain
Relationship Type: D - Direct protein target assigned
Confidence: Direct single protein target assigned

External ID: CHEMBL2444911

Protocol: N/A

Comment: Journal: Bioorg. Med. Chem.
Year: 2013
Volume: 21
Issue: 22
First Page: 7107
Last Page: 7117
DOI: 10.1016/j.bmc.2013.09.010

Target ChEMBL ID: CHEMBL612542
ChEMBL Target Name: SiHa
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular

External ID: CHEMBL2444910

Protocol: N/A

Comment: Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation.

Journal: Bioorg. Med. Chem.
Year: 2013
Volume: 21
Issue: 22
First Page: 7107
Last Page: 7117
DOI: 10.1016/j.bmc.2013.09.010

Target ChEMBL ID: CHEMBL612542
ChEMBL Target Name: SiHa
ChEMBL Target Type: CELL-LINE - Target is a specific cell-line
Relationship Type: N - Non-molecular target assigned
Confidence: Target assigned is non-molecular