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酸性红52

酸性红52用途

Sulforhodamine B sodium salt 是一种荧光染料,可用于定量测定培养细胞的细胞蛋白量。

酸性红52名称

[ CAS 号 ]:
3520-42-1

[ 中文名 ]:
丽丝胺罗丹明B

[ 英文名 ]:
Acid Red 52

[中文别名 ]:

[英文别名 ]:

4'-Butoxy-3-piperidinopropiophenone hydrochloride
Tanaclone
EINECS 222-529-8
dyclonine hydrochloride
SULFORHODAMINE B
MFCD00010180
C.I. 45100
dye acid red 52
Sulforhodamine-B
Dyclone

酸性红52生物活性

[ 描述 ]:

Sulforhodamine B sodium salt 是一种荧光染料,可用于定量测定培养细胞的细胞蛋白量。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

磺酰罗丹明B(SRB)通常用作膜不可渗透的极性示踪剂或通过测定细胞蛋白用于细胞密度测定(细胞毒性测定)。 SRB测定已被用于廉价地进行各种筛选测定以研究基于细胞的研究中的细胞毒性。该方法依赖于SRB的性质,SRB在温和的酸性条件下以化学计量方式与蛋白质结合,然后可以使用碱性条件提取;因此,结合染料的量可以用作细胞质量的代表,然后可以外推以测量细胞增殖。该方案可分为四个主要步骤：处理的准备,用选择的处理孵育细胞,细胞固定和SRB染色,以及吸光度测量。该测定仅限于手动或半自动筛选,并且可以以有效且灵敏的方式用于测试贴壁细胞中的化学治疗药物或小分子。它还可用于评估基因表达调节(敲低,基因表达上调)的影响,以及研究miRNA替代对细胞增殖的影响[1]。

[细胞实验]

轻轻地向每个孔中加入25μL（96孔格式）或5μL（384孔格式）冷50％（wt / vol）TCA至培养基上清液，并将板在4℃下孵育1小时。不需要混合，因为这可能导致一些细胞从孔底部分离。将板浸入带有慢速自来水的桶中，将板洗净四次，然后轻轻地将板轻拍到纸巾中，除去多余的水。最后一次洗涤后，让板在室温下风干。向每个孔中加入50μL（96孔格式）或20μL（384孔格式）0.04％（wt / vol）SRB溶液。在室温下放置1小时，然后用1％（体积/体积）乙酸（200μL用于96孔格式或30μL用于384孔格式）快速冲洗板四次以除去未结合的染料。让板在室温下风干[1]。

[参考文献]

[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.

[相关活性小分子]

酸性红52物理化学性质

[ 分子式 ]:
C₂₇H₂₉N₂NaO₇S₂

[ 分子量 ]:
580.65

[ PSA ]:
150.78000

[ LogP ]:
7.81310

[ 外观性状 ]:
粉末

[ 储存条件 ]:
室温，避光，密封

[ 水溶解性 ]:
methanol: 1 mg/mL

酸性红52MSDS

详细MSDS(安全技术说明书)

酸性红52毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: BP6750000

CHEMICAL NAME :: Ammonium, (6-(diethylamino)-9-(2,4-disulfophenyl)-3H-xanthen-3- ylidene)diethyl-, hydroxide, inner salt, sodium salt

CAS REGISTRY NUMBER :: 3520-42-1

LAST UPDATED :: 199701

DATA ITEMS CITED :: 5

MOLECULAR FORMULA :: C27-H29-N2-O7-S2.Na

MOLECULAR WEIGHT :: 580.69

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 10300 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TYPE OF TEST :: Cytogenetic analysis

TEST SYSTEM :: Rodent - hamster Fibroblast

DOSE/DURATION :: 13100 mg/L

REFERENCE :: FCTOD7 Food and Chemical Toxicology. (Pergamon Press Inc., Maxwell House, Fairview Park, Elmsford, NY 10523) V.20- 1982- Volume(issue)/page/year: 22,623,1984 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M2208 No. of Facilities: 114 (estimated) No. of Industries: 5 No. of Occupations: 7 No. of Employees: 901 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M2208 No. of Facilities: 2248 (estimated) No. of Industries: 47 No. of Occupations: 51 No. of Employees: 75084 (estimated) No. of Female Employees: 12598 (estimated)

酸性红52安全信息

[ 符号 ]:

GHS07

[ 信号词 ]:
Warning

[ 危害声明 ]:
H315-H319-H335

[ 警示性声明 ]:
P261-P305 + P351 + P338

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xi

[ 风险声明 (欧洲) ]:
36/37/38

[ 安全声明 (欧洲) ]:
22-24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
2

[ RTECS号 ]:
BP6750000

[ 海关编码 ]:
3204120000

酸性红52上下游产品

酸性红52上游产品

酸性红52下游产品

酸性红52制备

1.主要采用缩合法。以间羟基二乙基苯胺为原料，与间二磺酸基苯甲醛进行缩合反应，然后经氧化、过滤、干燥、粉碎而得。

酸性红52海关

[ 海关编码 ]: 2921499090

[ 中文概述 ]:
2921499090 其他芳香单胺及衍生物及它们的盐. 增值税率:17.0% 退税率:9.0% 监管条件:无最惠国关税:6.5% 普通关税:30.0%

[ 申报要素 ]: 品名, 成分含量, 用途

[ Summary ]:
2921499090 other aromatic monoamines and their derivatives; salts thereof VAT:17.0% Tax rebate rate:9.0% Supervision conditions:none MFN tariff:6.5% General tariff:30.0%

酸性红52文献

The dynamics of giant unilamellar vesicle oxidation probed by morphological transitions.

Biochim. Biophys. Acta 1838(10) , 2615-24, (2014)

We have studied the dynamics of Lissamine Rhodamine B dye sensitization-induced oxidation of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) giant unilamellar vesicles (GUVs), where the progression of...

Enhancing the anti-inflammatory activity of chalcones by tuning the Michael acceptor site.

Org. Biomol. Chem. 13(10) , 3040-7, (2015)

Inflammatory signaling pathways orchestrate the cellular response to infection and injury. These pathways are known to be modulated by compounds that alkylate cysteinyl thiols. One class of phytochemi...

Enhancing perception of contaminated food through acid-mediated modulation of taste neuron responses.

Curr. Biol. 24(17) , 1969-77, (2014)

Natural foods contain not only nutrients, but also nonnutritious and potentially harmful chemicals. Thus, animals need to evaluate food content in order to make adequate feeding decisions.Here, we inv...

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