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4-硝基苯基-β-D-纤维二糖苷

4-硝基苯基-β-D-纤维二糖苷用途

4-硝基苯基β-D-纤维糖苷(对硝基苯基β-D纤维糖苷)是一种纤维三糖类似物,是检测纤维素酶活性的显色底物。外葡聚糖酶、内葡聚糖酶和β-葡萄糖苷酶水解4-硝基苯基β-D-纤维素糖苷,生成对硝基苯酚(PNP)[1][2]。

4-硝基苯基-β-D-纤维二糖苷名称

[ CAS 号 ]:
3482-57-3

[ 中文名 ]:
4-硝基苯基-β-D-纤维二糖苷

[ 英文名 ]:
4-Nitrophenyl β-D-Cellobioside

[中文别名 ]:

[英文别名 ]:

4-NITROPHENYL-β-D-CELLOBIOSIDE
4-Nitrophenyl β-D-Cellobioside
4-NITROPHENYL-BETA-D-CELLOBIOSIDE
4-Nitrophenyl beta-D-Cellobioside
MFCD00069845

4-硝基苯基-β-D-纤维二糖苷生物活性

[ 描述 ]:

[ 相关类别 ]:

研究领域 >> 其他

信号通路 >> 其他 >> 其他

[参考文献]

[1]. Coleman DJ, et al. A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-beta-D-cellobioside. Anal Biochem. 2007 Dec 15;371(2):146-53.

[2]. Yukihiko Hayashi, et al. Transcellobiosylation Reactions Catalyzed by Different Exoglucanase Components of a T richoderma viride Cellulase in Aqueous Organic Solvent. Biocatal Biotransfor, 2003, 21(1): 25-31.

4-硝基苯基-β-D-纤维二糖苷物理化学性质

[ 密度 ]:
1.7 g/cm3

[ 沸点 ]:
795.6ºC at 760 mmHg

[ 熔点 ]:
249-250ºC

[ 分子式 ]:
C₁₈H₂₅NO₁₃

[ 分子量 ]:
463.39000

[ 闪点 ]:
435ºC

[ 精确质量 ]:
463.13300

[ PSA ]:
224.35000

[ 外观性状 ]:
灰白色粉末

[ 折射率 ]:
1.67

[ 储存条件 ]:
2-8°C

[ 水溶解性 ]:
methanol: water (2:3): 50 mg/mL, clear, faintly yellow

4-硝基苯基-β-D-纤维二糖苷MSDS

4-硝基苯基-β-D-纤维二糖苷安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 安全声明 (欧洲) ]:
S24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ 海关编码 ]:
29400090

4-硝基苯基-β-D-纤维二糖苷合成路线

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4-硝基苯基-β-D-纤维二糖苷文献

Mechanistic studies of active site mutants of Thermomonospora fusca endocellulase E2.

Biochemistry 38(30) , 9746-51, (1999)

Endocellulase E2 from the thermophilic bacterium Thermomonospora fusca is a member of glycosyl-hydrolase family 6 and is active from pH 4 to 10. Enzymes in this family hydrolyze beta-1,4-glycosidic bo...

Structural changes of cellobiohydrolase I (1,4-beta-D-glucan-cellobiohydrolase I, CBHI) and PNPC (p-nitrophenyl-beta-D-cellobioside) during the binding process.

Sci. China,. C, Life Sci. 51(5) , 459-69, (2008)

Conformational changes to 1,4-beta-D-glucan cellobiohydrolase I (CBHI) in response to its binding with p-nitrophenyl beta-D-cellobioside (PNPC) were analyzed by second-derivative fluorescence spectrom...

The competitive inhibition of Trichoderma reesei C30 cellobiohydrolase I by guanidine hydrochloride.

FEBS Lett. 270(1-2) , 143-6, (1990)

The p-nitrophenylcellobiosidase (PNPCase) activity of Trichoderma reesei cellobiohydrolase I (CBH I) was competitively inhibited by concentrations of guanidine hydrochloride (Gdn HCl) that did not aff...

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