Muconomycin A

Muconomycin A用途

Verrucarin A (Muconomycin A) 是一种 D 型大环真菌毒素,来源于Myrothecium verrucaria,是一种蛋白质合成 (protein synthesis) 的抑制剂。Verrucarin A 抑制白血病细胞系生长,激活巨噬细胞 caspases、凋亡和炎症信号。Verrucarin A 能有效提高 p38 MAPK 的磷酸化,降低 ERK/Akt 的磷酸化。Verrucarin A 通过 p21 和 p53 的诱导引起细胞周期调控的解除。

Muconomycin A名称

[ CAS 号 ]:
3148-09-2

[ 中文名 ]:
疣孢菌素A

[ 英文名 ]:
Verrucarin A

[中文别名 ]:

疣孢菌素

[英文别名 ]:

Muconomycin A
Antibiotic 379Y
VER A
Verrucarin A

Muconomycin A生物活性

[ 描述 ]:

[ 相关类别 ]:

信号通路 >> 细胞凋亡 >> 细胞凋亡

研究领域 >> 癌症

[体外研究]

疣甲素(0-0.6μM/ml；24-48小时)-诱导MCF-7细胞的时间和剂量依赖性生长抑制[1]。疣甲素增加活性氧(ROS)水平,导致线粒体膜电位(Δψm)丢失,导致Bax/Bcl-2比值增加、细胞色素c释放、caspase活化、PARP降解和细胞凋亡[1]。细胞活力测定[1]细胞系：MCF-7细胞浓度：0-0.6μM/ml孵育时间：24小时和48小时结果：MCF-7细胞生长受到明显抑制,24小时和48小时的IC50s分别为0.41和0.29μM/ml。

[参考文献]

[1]. Palanivel K, et al. Verrucarin A alters cell-cycle regulatory proteins and induces apoptosis through reactive oxygen species-dependent p38MAPK activation in the human breast cancer cell line MCF-7. Tumour Biol. 2014;35(10):10159-10167.

[2]. Palanivel K, et al. Verrucarin A, a protein synthesis inhibitor, induces growth inhibition and apoptosis in breast cancer cell lines MDA-MB-231 and T47D. Biotechnol Lett. 2013;35(9):1395-1403.

Muconomycin A物理化学性质

[ 密度 ]:
1.32g/cm3

[ 沸点 ]:
747.4ºC at 760mmHg

[ 熔点 ]:
>360ºC

[ 分子式 ]:
C₂₆H₃₂O₉

[ 分子量 ]:
488.52700

[ 闪点 ]:
250.2ºC

[ 精确质量 ]:
488.20500

[ PSA ]:
120.89000

[ LogP ]:
1.78050

[ 外观性状 ]:
固体

[ 折射率 ]:
1.584

[ 储存条件 ]:
2-8°C，密封，干燥

Muconomycin A毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: WH1314900

CHEMICAL NAME :: Spiro(16,18-methano-1H,3H,23H-(1,6,12)trioxacycloocta decino(3,4-d)(1)benzo pyran-17(18H),2'- oxirane)-3,9,14-trione, 4,5,6,7,16,16a,19a,22-octahydro-4-hydroxy-5,16a,21-tr imethyl-, stereoisomer

CAS REGISTRY NUMBER :: 3148-09-2

LAST UPDATED :: 199209

DATA ITEMS CITED :: 9

MOLECULAR FORMULA :: C27-H34-O9

MOLECULAR WEIGHT :: 502.61

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: Standard Draize test

ROUTE OF EXPOSURE :: Administration onto the skin

SPECIES OBSERVED :: Rodent - guinea pig

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 870 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 500 ug/kg

TOXIC EFFECTS :: Skin and Appendages - dermatitis, other (after systemic exposure) Biochemical - Metabolism (Intermediary) - effect on inflammation or mediation of inflammation

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Subcutaneous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 3900 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 1500 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 540 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 14 mg/kg/8W-C

TOXIC EFFECTS :: Blood - changes in leukocyte (WBC) count

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Primate - monkey

DOSE/DURATION :: 5 mg/kg/50D-C

TOXIC EFFECTS :: Blood - leukopenia Blood - changes in cell count (unspecified) Related to Chronic Data - death

MUTATION DATA

TYPE OF TEST :: DNA inhibition

TEST SYSTEM :: Rodent - mouse Lymphocyte

DOSE/DURATION :: 1 nmol/L

REFERENCE :: PLMEAA Planta Medica. (Georg Thieme Verlag, Postfach 732, D-7000 Stuttgart 1, Fed. Rep. Ger.) V.1- 1953- Volume(issue)/page/year: 34,231,1978

Muconomycin A安全信息

[ 符号 ]:

GHS06

[ 信号词 ]:
Danger

[ 危害声明 ]:
H300 + H310 + H330

[ 警示性声明 ]:
P260-P264-P280-P284-P301 + P310-P302 + P350

[ 个人防护装备 ]:
Eyeshields;Faceshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges

[ 危害码 (欧洲) ]:
T+: Very toxic;

[ 风险声明 (欧洲) ]:
26/27/28

[ 危险品运输编码 ]:
UN 2811 6.1/PG 1

[ WGK德国 ]:
3

[ RTECS号 ]:
WH1314900

[ 包装等级 ]:
I

[ 危险类别 ]:
6.1(a)