多聚-D-赖氨酸氢溴酸盐

多聚-D-赖氨酸氢溴酸盐用途

聚-D-赖氨酸聚合物可用于制备细胞附着的表面。D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞。建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。该产品是一种细胞的非特异性附着因子，可用于通过增强细胞膜的带负电离子与培养表面之间的静电相互作用而促进细胞与固体基质的粘附。当被细胞培养表面吸收后，聚-D-赖氨酸可增加带正电的细胞结合位点数量。聚-D-赖氨酸是一种带正电的氨基酸聚合物，其中每个赖氨酸残基约有一个HBr。氢溴酸盐可使聚-D-赖氨酸呈可溶于水的结晶形式。在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。

多聚-D-赖氨酸氢溴酸盐名称

[ CAS 号 ]:
27964-99-4

[ 中文名 ]:
多聚-D-赖氨酸氢溴酸盐

[ 英文名 ]:
Poly-D-lysine hydrobromide (MW 30000-70000)

[英文别名 ]:

MFCD00131934
Lysine hydrobromide (1:1)
Lysine, hydrobromide (1:1)

多聚-D-赖氨酸氢溴酸盐生物活性

[ 描述 ]:

聚D-赖氨酸氢溴酸盐(MW 30000-70000)是一种合成聚合物基质,是神经细胞培养中应用最广泛的基质之一。聚D-赖氨酸氢溴酸盐(MW 30000-70000)从朊病毒感染的SN56神经母细胞瘤细胞中去除蛋白酶K抗性PrP,而不影响PrPC[1][2]。

[ 相关类别 ]:

研究领域 >> 癌症

信号通路 >> 其他 >> 其他

[体外研究]

聚D-赖氨酸仅在低剂量注射时对兔子具有免疫原性[1]。将PrPres量降低50%所需的聚-D-赖氨酸(PDL)的近似浓度相当于约0.8μg/mL或50 nM[2]。

[参考文献]

[1]. HansVermeersch, et al. Immunogenicity of poly-D-lysine, a potential polymeric drug carrier. Journal of Controlled Release. Volume 32, Issue 3, 1 December 1994, Pages 225-229.

[2]. Zhou Xu, et al. Mechanistic insights into cellular alteration of prion by poly-D-lysine: the role of H2H3 domain. FASEB J. 2011 Oct;25(10):3426-35.

多聚-D-赖氨酸氢溴酸盐物理化学性质

[ 沸点 ]:
343.7±0.0 °C at 760 mmHg

[ 分子式 ]:
(C₆H₁₄N₂O₂)x_.xBrH

[ 分子量 ]:
30000-70000

[ 闪点 ]:
161.7±0.0 °C

[ 精确质量 ]:
226.031677

[ 外观性状 ]:
lyophilized powder

[ 蒸汽压 ]:
0.0±0.0 mmHg at 25°C

[ 储存条件 ]:
−20°C

[ 水溶解性 ]:
Soluble in water.

多聚-D-赖氨酸氢溴酸盐安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

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