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荧光素二(β-D-吡喃半乳糖苷)

荧光素二(β-D-吡喃半乳糖苷)用途

Fluorescein di(β-D-galactopyranoside) 是 β-半乳糖苷酶的荧光底物 (λex=485 nm,λem=535 nm)。

荧光素二(β-D-吡喃半乳糖苷)名称

[ CAS 号 ]:
17817-20-8

[ 中文名 ]:
荧光素二(beta-D-吡喃半乳糖苷)

[ 英文名 ]:
Fluorescein di(β-D-galactopyranoside)

[中文别名 ]:

[英文别名 ]:

荧光素二(β-D-吡喃半乳糖苷)生物活性

[ 描述 ]:

Fluorescein di(β-D-galactopyranoside) 是 β-半乳糖苷酶的荧光底物 (λex=485 nm,λem=535 nm)。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

荧光素二(β-D-吡喃半乳糖苷)产生的荧光以时间和剂量依赖性方式增加。双底物法产生的荧光水平远低于荧光素二(β-D-吡喃半乳糖苷)法产生的荧光水平。结果表明荧光素二(β-D-吡喃半乳糖苷)在Hs68细胞中产生的荧光素与传代次数成正比[1]。

[细胞实验]

将细胞（每孔5×103个细胞）在96孔板中培养过夜以进行附着，洗涤，然后在溶液中固定。将等分试样（100μL）的反应缓冲液（即，没有X-Gal的染色溶液）加入每个孔中。然后，每孔加入10μL2mM荧光素二（β-D-吡喃半乳糖苷），将板在黑暗中于37℃温育24小时，不加入CO 2。在37℃温育24小时后，将100μL上清液转移到96孔板中，一式三份进行荧光测量。使用荧光计测量荧光素荧光，激发波长为485nm，发射波长为535nm [1]。

[参考文献]

[1]. Yang NC, et al. A fluorimetric method using fluorescein di-beta-D-galactopyranoside for quantifying the senescence-associated beta-galactosidase activity in human foreskin fibroblast Hs68 cells. Anal Biochem. 2004 Feb 15;325(2):337-43.

[相关活性小分子]

荧光素二(β-D-吡喃半乳糖苷)物理化学性质

[ 密度 ]:
1.74 g/cm3

[ 沸点 ]:
974.8ºC at 760 mmHg

[ 熔点 ]:
200-203ºC (dec.)

[ 分子式 ]:
C₃₂H₃₂O₁₅

[ 分子量 ]:
656.58700

[ 闪点 ]:
316.5ºC

[ 精确质量 ]:
656.17400

[ PSA ]:
234.29000

[ 外观性状 ]:
黄色粉末

[ 蒸汽压 ]:
0mmHg at 25°C

[ 折射率 ]:
1.764

[ 储存条件 ]:
−20°C

荧光素二(β-D-吡喃半乳糖苷)安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 安全声明 (欧洲) ]:
22-24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ 海关编码 ]:
32129000

荧光素二(β-D-吡喃半乳糖苷)文献

High-throughput screening with nanoimprinting 3D culture for efficient drug development by mimicking the tumor environment.

Biomaterials 51 , 278-89, (2015)

Anti-cancer drug development typically utilizes high-throughput screening with two-dimensional (2D) cell culture. However, 2D culture induces cellular characteristics different from tumors in vivo, re...

Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis.

Appl. Microbiol. Biotechnol. 99 , 5639-46, (2015)

DNA damage is the dominant source of mutation, which is the driving force of evolution. Therefore, it is important to quantitatively analyze the DNA damage caused by different mutagenesis methods, the...

A programmable microfluidic static droplet array for droplet generation, transportation, fusion, storage, and retrieval.

Lab Chip 15 , 3677-86, (2015)

We present a programmable microfluidic static droplet array (SDA) device that can perform user-defined multistep combinatorial protocols. It combines the passive storage of aqueous droplets without an...

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