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罗丹明800

罗丹明800用途

Rhodamine 800 是一种近红外荧光染料。

罗丹明800名称

[ CAS 号 ]:
137993-41-0

[ 中文名 ]:
罗丹明800

[ 英文名 ]:
Rhodamine 800

[英文别名 ]:

罗丹明800生物活性

[ 描述 ]:

Rhodamine 800 是一种近红外荧光染料。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

罗丹明800在各种有机溶剂和可见光和近红外区域的水溶液中具有不同的光学吸收光谱。丙酮中683nm处的最大吸收在DMSO中转变为696nm而没有整体光谱分布的显着变化。罗丹明800的荧光强度随溶剂的变化而显着变化,当溶剂从丙酮变为水而没有发射峰的显着变化时,罗丹明800的荧光强度降低至不到五分之一[1]。

[激酶实验]

将罗丹明800（Rh800）和吲哚菁绿（ICG）溶解在PBS中，并将它们的浓度设定为40nM。向3mL罗丹明800溶液（40nM / PBS）中加入100μLp-sulfonatocalix [n]芳烃（S [n]）（1mM）。在搅拌下向该溶液中加入微升等分的乙酰胆碱（ACh）（350mM）。搅拌2分钟后，测量荧光光谱。对于其他神经递质，氨基酸，氯化铵和胆碱的竞争性荧光团置换，应用与上述方法类似的程序[2]。

[参考文献]

[1]. Sakanoue J, et al. Rhodamine 800 as a probe of energization of cells and tissues in the near-infrared region: a study with isolated rat liver mitochondria and hepatocytes. J Biochem. 1997 Jan;121(1):29-37.

[2]. Jin T. et al. Near-infrared fluorescence detection of acetylcholine in aqueous solution using a complex ofrhodamine 800 and p-sulfonatocalix[8]arene. Sensors (Basel). 2010;10(3):2438-49.

[相关活性小分子]

罗丹明800物理化学性质

[ 分子式 ]:
C₂₆H₂₆ClN₃O₅

[ 分子量 ]:
495.955

[ 精确质量 ]:
495.156097

[ 外观性状 ]:
固体;Purple to Dark purple to Brown powder to crystal

[ 储存条件 ]:
室温

罗丹明800安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危险品运输编码 ]:
NONH for all modes of transport

罗丹明800文献

Flow cytometry of DNA content using oxazine 750 or related laser dyes with 633 nm excitation.

Cytometry 7 , 107, (1986)

The laser dyes oxazine 750 (OX750), LD700, and rhodamine 800 (R800) can be used in an instrument employing a low-power helium-neon laser source for flow cytometry of DNA content in ethanol-fixed or de...

The effect of varying short-chain alkyl substitution on the molar absorptivity and quantum yield of cyanine dyes.

Anal. Chem. Insights 6 , 29-36, (2011)

The effect of varying short-chain alkyl substitution of the indole nitrogens on the spectroscopic properties of cyanine dyes was examined. Molar absorptivities and fluorescence quantum yields were det...

Near-infrared fluorescence detection of acetylcholine in aqueous solution using a complex of rhodamine 800 and p-sulfonatocalix[8]arene.

Sensors (Basel.) 10 , 2438-49, (2012)

The complexing properties of p-sulfonatocalix[n]arenes (n = 4: S[4], n = 6: S[6], and n = 8: S[8]) for rhodamine 800 (Rh800) and indocyanine green (ICG) were examined to develop a near-infrared (NIR) ...

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