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溴化乙啶

溴化乙啶用途

Ethidium bromide 常用作分子生物实验学中的荧光标记(核酸染色),如琼脂糖凝胶电泳。

溴化乙啶名称

[ CAS 号 ]:
1239-45-8

[ 中文名 ]:
溴化乙啶

[ 英文名 ]:
Ethidium bromide

[中文别名 ]:

[英文别名 ]:

3,8-Diamino-5
MFCD00011724
Homidium bromide
EINECS 214-984-6
EthidiuM BroMide

溴化乙啶生物活性

[ 描述 ]:

Ethidium bromide 常用作分子生物实验学中的荧光标记(核酸染色),如琼脂糖凝胶电泳。

[ 相关类别 ]:

研究领域 >> 其他

信号通路 >> 其他 >> 其他

[体外研究]

指南(以下是我们推荐的方案。本协议仅提供指南,应根据您的具体需要进行修改)。琼脂糖凝胶的制备：1.琼脂糖凝胶的浓度一般为0.5%-2.5%,取决于需要分离的条带大小。2.将琼脂糖粉与1X TAE/TBE混合。3.微波加热1-3分钟,直到琼脂糖完全溶解(注意：不要过度搅拌)。4.使溶液冷却后再凝固。5.添加溴化乙锭(EtBr),最终浓度约为0.2-0.5μg/mL(储备通常为10 mg/mL,需要5µL储备/100 mL凝胶)。6.溴化乙锭与DNA结合,你可以在紫外线下看到DNA。注意：EtBr是已知的诱变剂。请注意加强保护。

[参考文献]

[1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84.

[2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0.

溴化乙啶物理化学性质

[ 熔点 ]:
260-262 °C (dec.)(lit.)

[ 分子式 ]:
C₂₁H₂₀BrN₃

[ 分子量 ]:
394.308

[ 闪点 ]:
>100°C

[ 精确质量 ]:
393.084045

[ PSA ]:
55.92000

[ LogP ]:
2.29820

[ 外观性状 ]:
粉末

[ 折射率 ]:
1.4575 (20ºC)

[ 储存条件 ]:
2-8°C

[ 稳定性 ]:
Stable. Incompatible with strong oxidizing agents.

[ 水溶解性 ]:
40 g/L (25 ºC)

溴化乙啶MSDS

详细MSDS(安全技术说明书)

溴化乙啶毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SF7950000

CHEMICAL NAME :: Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide

CAS REGISTRY NUMBER :: 1239-45-8

LAST UPDATED :: 199712

DATA ITEMS CITED :: 79

MOLECULAR FORMULA :: C21-H20-N3.Br

MOLECULAR WEIGHT :: 394.35

WISWESSER LINE NOTATION :: T B666 HKJ EJ H2 IR& LZ &E &9/26

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 20 mg/kg

TOXIC EFFECTS :: Tumorigenic - active as anti-cancer agent

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Subcutaneous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 110 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: Sex chromosome loss and nondisjunction

TYPE OF TEST :: Specific locus test

TYPE OF TEST :: Dominant lethal test

TYPE OF TEST :: Micronucleus test

TYPE OF TEST :: DNA inhibition

TYPE OF TEST :: Sister chromatid exchange

MUTATION DATA

TEST SYSTEM :: Bird - chicken

DOSE/DURATION :: 847 ug/kg

REFERENCE :: 47JMAE "Cytogenetic Assays of Environmental Mutagens," Hsu, T.C., ed., Totowa, NJ, Allanheld, Osmun & Co., 1982 Volume(issue)/page/year: -,137,1982 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - A1168 No. of Facilities: 17 (estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees: 34 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - A1168 No. of Facilities: 7 (estimated) No. of Industries: 1 No. of Occupations: 3 No. of Employees: 21 (estimated)

溴化乙啶安全信息

[ 符号 ]:

GHS06, GHS08

[ 信号词 ]:
Danger

[ 危害声明 ]:
H302-H330-H341

[ 警示性声明 ]:
P260-P281-P284-P310

[ 个人防护装备 ]:
Eyeshields;Faceshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges

[ 危害码 (欧洲) ]:
T+:Verytoxic;

[ 风险声明 (欧洲) ]:
R21/22;R26;R36/37/38;R68

[ 安全声明 (欧洲) ]:
S36/37-S45-S36/37/39-S28A-S26-S22

[ 危险品运输编码 ]:
UN 2811 6.1/PG 1

[ WGK德国 ]:
3

[ RTECS号 ]:
SF7950000

[ 包装等级 ]:
I

[ 危险类别 ]:
6.1

[ 海关编码 ]:
29339990

溴化乙啶合成路线

详细合成路线

溴化乙啶上下游产品

溴化乙啶上游产品

溴化乙啶下游产品

溴化乙啶海关

[ 海关编码 ]: 2933990090

[ 中文概述 ]:
2933990090. 其他仅含氮杂原子的杂环化合物. 增值税率:17.0%. 退税率:13.0%. 监管条件:无. 最惠国关税:6.5%. 普通关税:20.0%

[ 申报要素 ]: 品名, 成分含量, 用途, 乌洛托品请注明外观, 6－己内酰胺请注明外观, 签约日期

[ Summary ]:
2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

溴化乙啶文献

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Int. J. Food Microbiol. 189 , 98-105, (2014)

The aim of this study was to analyze the changing patterns of Listeria monocytogenes contamination in a cheese processing facility manufacturing a wide range of ready-to-eat products. Characterization...

Alpha-fetoprotein, identified as a novel marker for the antioxidant effect of placental extract, exhibits synergistic antioxidant activity in the presence of estradiol.

PLoS ONE 9(6) , e99421, (2014)

Placenta, as a reservoir of nutrients, has been widely used in medical and cosmetic materials. Here, we focused on the antioxidant properties of placental extract and attempted to isolate and identify...

Vegetables' juice influences polyol pathway by multiple mechanisms in favour of reducing development of oxidative stress and resultant diabetic complications.

Pharmacogn. Mag. 10(Suppl 2) , S383-91, (2014)

Hyperglycemia induced generation of free radicals and consequent development of oxidative stress by polyol pathway is one of the crucial mechanisms stirring up development of diabetic complications. W...

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