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4-(6-羧基-2-吲哚基)-4'-甲基-2,2'-(乙烯基二氧)二苯胺-N,N,N',N'-四乙酸四(乙酰氧基甲基)酯

用途

Indo-1 AM 是一种荧光 Ca²⁺ 指示剂 (λ_ex=340 nm，λ_em=405/485 nm)。

名称

[ CAS 号 ]:
112926-02-0

[ 中文名 ]:
钙荧光探针 Indo 1-AM

[ 英文名 ]:
Indo-1 AM

[中文别名 ]:

[英文别名 ]:

MFCD00036955
Indo-1 pentaacetoxymethyl ester
INDO 1/AM
Indo-1 AM

生物活性

[ 描述 ]:

Indo-1 AM 是一种荧光 Ca2+ 指示剂 (λex=340 nm,λem=405/485 nm)。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

Indo-1 AM是荧光Ca2 +指示剂,λex= 340 nm,λem= 405/485 nm [1]。在暴露于Indo-1 AM的兔心脏中,72％的染料存在于胞质溶胶中,其余的与线粒体,微粒体和细胞核相关[2]。

[细胞实验]

通过分别暴露于膜透过形式Indo-1AM（吲哚-1-乙酰氧基甲酯）和呋喃-2-乙酰氧基甲酯，使细胞负载indo-I和fura-2。 10μL1mMIndo-1 AM或fura-2 / AM在二甲基亚砜（DMSO）中的混合物，2.5μL含25％wt / wt Pluronic F-127的DMSO溶液和75μL胎牛血清（FCS）或新生儿将小牛血清（NCS）加入到含有细胞和40μLFCS或NCS的2mL Tyrode溶液中。通过轻轻摇动细胞15至30分钟来实现加载[2]。

[参考文献]

[1]. Andrienko T, et al. Real-Time Fluorescence Measurements of ROS and [Ca2+] in Ischemic / Reperfused Rat Hearts: Detectable Increases Occur only after Mitochondrial Pore Opening and Are Attenuated by Ischemic Preconditioning. PLoS One. 2016 Dec 1;11(12):e0167300.

[2]. Blatter LA, et al. Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2. Biophys J. 1990 Dec;58(6):1491-9.

[相关活性小分子]

物理化学性质

[ 密度 ]:
1.374g/cm3

[ 沸点 ]:
1009.2ºC at 760mmHg

[ 分子式 ]:
C₄₇H₅₁N₃O₂₂

[ 分子量 ]:
1009.91000

[ 闪点 ]:
564.1ºC

[ 精确质量 ]:
1009.30000

[ PSA ]:
303.73000

[ LogP ]:
3.14650

[ 蒸汽压 ]:
3.2E-28mmHg at 25°C

[ 折射率 ]:
1.577

[ 储存条件 ]:
-20°C，密闭，干燥

安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 安全声明 (欧洲) ]:
S22-S24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

文献

Quantitative TCR:pMHC Dissociation Rate Assessment by NTAmers Reveals Antimelanoma T Cell Repertoires Enriched for High Functional Competence.

J. Immunol. 195 , 356-66, (2015)

Experimental models demonstrated that therapeutic induction of CD8 T cell responses may offer protection against tumors or infectious diseases providing that T cells have sufficiently high TCR/CD8:pMH...

Identification of Rare High-Avidity, Tumor-Reactive CD8+ T Cells by Monomeric TCR-Ligand Off-Rates Measurements on Living Cells.

Cancer Res. 75 , 1983-91, (2015)

The avidity of the T-cell receptor (TCR) for antigenic peptides presented by the peptide-MHC (pMHC) on cells is a key parameter for cell-mediated immunity. Yet a fundamental feature of most tumor anti...

Pharmacological analysis by HOE642 and KB-R9032 of the role of Na(+)/H(+) exchange in the endothelin-1-induced Ca(2+) signalling in rabbit ventricular myocytes.

Br. J. Pharmacol. 131(3) , 638-44, (2000)

The role of Na(+)/H(+) exchange in endothelin-1 (ET-1)-induced increases in Ca(2+) transients and cell shortening was studied in rabbit ventricular myocytes loaded with indo-1/AM. Selective inhibitors...

更多文献

4-(6-羧基-2-吲哚基)-4'-甲基-2,2'-(乙烯基二氧)二苯胺-N,N,N',N'-四乙酸四(乙酰氧基甲基)酯

用途

名称

生物活性

物理化学性质

安全信息

文献

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