Biochemistry

Structural and mechanistic studies of HpxO, a novel flavin adenine dinucleotide-dependent urate oxidase from Klebsiella pneumoniae

KA Hicks, SE O'Leary, TP Begley, SE Ealick

文献索引：Hicks, Katherine A.; O'Leary, Sean E.; Begley, Tadhg P.; Ealick, Steven E. Biochemistry, 2013 , vol. 52, # 3 p. 477 - 487

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被引用次数: 9

摘要

HpxO is a flavin-dependent urate oxidase that catalyzes the hydroxylation of uric acid to 5- hydroxyisourate and functions in a novel pathway for purine catabolism found in Klebsiella pneumoniae. We have determined the structures of HpxO with and without uric acid at 2.0 and 2.2 Å, respectively. We have also determined the structure of the R204Q variant at 2.0 Å resolution in the absence of uric acid. The variant structure is very similar to that of wild- ...