Photochemical Nucleophile Mapping: Identification of Tyr311 Within the Catalytic Domain of Rabbit Muscle Glyceraldehyde??3??phosphate Dehydrogenase

Y Hatanaka, M Kaneda…

文献索引：Hatanaka, Yasumaru; Kaneda, Masaki; Tomohiro, Takenori Photochemistry and Photobiology, 2007 , vol. 83, # 1 p. 213 - 217

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被引用次数: 1

摘要

Abstract Photochemical mapping of nucleophiles in close proximity to the active site Cys149 of rabbit glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was demonstrated based on the nucleophilic aromatic photosubstitution reaction using two regioisomers of alkoxy- fluoro-nitro-substituted benzenes. Two photophores were covalently attached to the active site SH group of GAPDH and the protein was subjected to photolysis then to the cyanogen ...