FEBS Letters 2005-08-15

Regulation of uncoupling protein activity in phosphorylating potato tuber mitochondria.

Rachel Navet, Pierre Douette, Florence Puttine-Marique, Claudine M Sluse-Goffart, Wieslawa Jarmuszkiewicz, Francis E Sluse

文献索引：FEBS Lett. 579(20) , 4437-42, (2005)

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摘要

In isolated potato tuber mitochondria, palmitic acid (PA) can induce a H+ leak inhibited by GTP in the phosphorylating (state 3) respiration but not in the resting (state 4) respiration. The PA-induced H+ leak is constant when state 3 respiration is decreased by an inhibition of the succinate uptake with n-butyl malonate (nBM). We show that the efficiency of inhibition by GTP is decreased when state 3 respiration is progressively inhibited by antimycin A (AA) and is restored following subsequent addition of nBM. We propose that in phosphorylating potato tuber mitochondria, the redox state of ubiquinone, which can antagonistically be varied with AA and nBM, modulates inhibition of the PA-activated UCP-sustained H+ leak by GTP.