Chemosphere 2010-08-01

Secondary oxidation of cyclic 1,N2-propano and 1,N2-etheno-2'-deoxyguanosine DNA adducts. Consequences in oxidative stress biomarker development.

Stéphane Barbati, Aurélie Bonnefoy, Alain Botta, Serge Chiron

文献索引：Chemosphere 80(9) , 1081-7, (2010)

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摘要

This work is an attempt to investigate the chemical stability of 1,N2-propano-2'-deoxyguanosine (pdG-HNE) and 1,N2-etheno-2'-deoxyguanosine (epsilondG) DNA adducts against hydrolysis and upon oxidation reactions. It includes both kinetic issues together with proposed degradation pathways. While both chemicals are stable in the 3.5-9 pH range, the results suggest that pdG-HNE adduct is less prone to in vitro oxidative transformation than epsilondG adduct. EpsilondG and pdG-HNE behave differently upon hydroxyl radical and one electron oxidation reactions. The exocyclic ring of epsilondG is mainly affected by oxidative processes leading to the regeneration of 2'-deoxyguanosine (dG) while the integrity of the exocyclic ring is preserved for pdG-HNE. Consequently, pdG-HNE might be a better biomarker than epsilondG for monitoring oxidative stress during environmental or occupational exposures to chemicals. Understanding the in vitro routes of etheno and propano DNA adduct degradation would probably help to guide the development of analytical methodologies for the reliable detection of these endogenous adducts.Copyright (c) 2010 Elsevier Ltd. All rights reserved.