Tetrahedron

DNA affinity cleaving: Sequence specific cleavage of DNA by Distamycin-EDTA-Fe (II) and EDTA-distamycin Fe (II)

JS Taylor, PG Schultz, PB Dervan

文献索引：Taylor, John S.; Schultz, Peter G.; Dervan, Peter B. Tetrahedron, 1984 , vol. 40, # 3 p. 457 - 465

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被引用次数: 199

摘要

The attachment of EDTA· Fe (II) to distamycin changes the sequence specific DNA binding antibiotic into a sequence specific DNA cleaving molecule. We report the synthesis of EDTA- distamycin (ED) which has the metal chelator, EDTA, tethered to the carboxy terminus of the N-methylpyrrole tripeptide moiety of the antiobiotic, distamycin. EDTA-distamycin-Fe (II)(ED)· FeII at 10-6M concentration efficiently cleaves pBR322 DNA (10-5M in base pairs) in the ...