13 C Labelling and electrospray mass spectrometry reveal a de novo route for inositol biosynthesis in Leishmania donovani parasite

P Sahai, M Chawla, RA Vishwakarma

文献索引：Sahai, Parmeshwari; Chawla, Mamta; Vishwakarma, Ram A. Journal of the Chemical Society, Perkin Transactions 1, 2000 , # 8 p. 1283 - 1290

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被引用次数: 15

摘要

In this paper we provide evidence for the presence of a de novo route for myo-inositol biosynthesis in the protozoan parasite Leishmania donovani, by the application of 13C labelling and electrospray ionisation mass spectrometry (ESMS). For this myo-[1-13C] inositol is prepared from D-[6-13C] glucose and biosynthetically incorporated in the parasite promastigote cell culture. Biosynthetic phosphatidylinositol (PI) and its hydrolysis products ...