Nucleic Acids Research 2000-05-01

Highly efficient solid phase synthesis of oligonucleotide analogs containing phosphorodithioate linkages.

D C Capaldi, D L Cole, V T Ravikumar

文献索引：Nucleic Acids Res. 28(9) , E40, (2000)

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摘要

A triester method for the synthesis of deoxynucleoside phosphorodithioate dimers is described. The phosphorodithioate linkage is introduced using a new dithiophosphorylating reagent DPSE-SP(S)Cl(2)where DPSE = 2-diphenylmethylsilylethyl. This group is removed quickly using tetra-butylammonium fluoride leading to the quantitative formation of phosphorodithioate diesters uncontaminated with the corresponding phosphorothioates. The utility of this group is demonstrated by the synthesis of a penta-decathymidylic acid, [T(PS(2))T(PO(2))](7)T, which contains alternating phosphorodithioate/phosphate diester internucleotide linkages.