Chemical Communications 2011-10-07

Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays.

Yasuhiko Iwasaki, Tatsuya Ota

文献索引：Chem. Commun. (Camb.) 47(37) , 10329-31, (2011)

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摘要

In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol-ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG(4)10K-SH). Biotinylated HL-60 cells were selectively adhered onto an avidin-patterned surface with high viability.This journal is © The Royal Society of Chemistry 2011