Steroids 1991-12-01

Fluorometric labeling of tetrahydroprogesterones.

J C Touchstone, X Fang

文献索引：Steroids 56(12) , 601-5, (1991)

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摘要

7-Diethylaminocourmarin-3-carbohydrazide was used to label the ketone group of the tetrahydroprogesterones to form fluorescent derivatives with high sensitivity. The four isomeric 3-hydroxypregnanes separated readily on high-performance, thin-layer chromatography after derivatization. This separation was not possible with the underivatized isomers. Standards and steroids from biologic mixtures were separated and showed similar characteristics. The methods used are described.