Journal of Veterinary Medical Science 2008-08-01

Total glutathione and glutathione reductase in bovine erythrocytes and liver biopsy.

Mahmoud Rushdi Abd Ellah, Keiji Okada, Masanobu Goryo, Saori Kobayashi, Akihiro Oishi, Jun Yasuda

文献索引：J. Vet. Med. Sci. 70 , 861-864, (2008)

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摘要

The goal of the present study was to measure the total glutathione level and glutathione reductase activity in bovine erythrocytes and liver biopsy. Five cows were injected intraperitoneally with DL-ethionine (12.5 mg/kg B.W.), and two control cows were injected with normal saline (0.9% NaCl). Ultrasonography guided liver biopsy, and blood samples were collected at 0, 4, 7 and 10 days after injection. The hepatic total glutathione level was significantly increased on Days 7 (p<0.05) and 10 (p<0.01), and hepatic glutathione reductase activity was significantly increased on Days 4 (p<0.05), 7 (p<0.01) and 10 (p<0.01). There were insignificant changes in the erythrocytic total glutathione level. The present study demonstrated that liver biopsy is a valuable tool for detecting oxidative stress and for diagnosing hepatic dysfunction in cattle from the viewpoint of the status of glutathione and glutathione reductase.