Journal of Pharmaceutical and Biomedical Analysis 1985-01-01

Determination of the theophylline solubilizer salicylamide-O-acetic acid in serum and urine using high-performance liquid chromatography.

H U Schulz, E Kraas

文献索引：J. Pharm. Biomed. Anal. 3(5) , 469-75, (1985)

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摘要

A high-performance liquid chromatographic method for the determination of the theophylline solubilizer salicylamide-O-acetic acid has been developed in the range 0.5 to 10 microg/ml for human serum and 5 to 400 microg/ml for urine. Reversed-phase ion-pair chromatography was employed with tetrabutylammonium hydrogen sulphate as counterion and 2-nitrophenylacetic acid as internal standard. Preliminary pharmacokinetic single-dose studies show that the sensitivity and the selectivity of the assay are adequate to measure lower concentrations in the late beta-phase.